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BHQ1
A T
A T
G C
Phosphorothioate bond
G C
G C
C G
T A
Restriction endonuclease site
C G
A T
Fluorophore-dT CT GT
G A
TACGCCACCAGCTCC ACTACCACAAGTTTATATTCAGTCATTTTCAGCAGGCCTTATAATAAAAATAATGAAAATGTGACTATATTA
3’ ACGGATGCGGTGGTCGAGGTTGATGGTGTTCAAATATAAGTCAGTAAAAGTCGTCCGGAATATTATTTTTATTACTTTTACACTGATATAATCTTG5’
Figure S2 OCEAN ternary structure. At the selected reaction temperature, the target (in black) binds the anchor
(in dark gray) and its specific amplifier (in light gray) forming a 3-way DNA junction that contains a restriction
endonuclease site (in the rectangle). The underlined base represents the nucleotide potentially mutated.