Download Powerpoint - Blood Journal

T-cell immunity to Kaposi sarcoma–associated
herpesvirus: recognition of primary effusion lymphoma by
LANA-specific CD4+ T cells
by Shereen Sabbah, Ya Jankey Jagne, Jianmin Zuo, Thushan de Silva, Mohammad
M. Ahasan, Christian Brander, Sarah Rowland-Jones, Katie L. Flanagan, and
Andrew D. Hislop
Blood
Volume 119(9):2083-2092
March 1, 2012
©2012 by American Society of Hematology
KSHV latent antigen-specific ELISPOT analysis of PBMCs from KSHV seropositive and
seronegative donors.
Shereen Sabbah et al. Blood 2012;119:2083-2092
©2012 by American Society of Hematology
Characterization of LANA-specific CD4+ T-cell clones.
Shereen Sabbah et al. Blood 2012;119:2083-2092
©2012 by American Society of Hematology
LANA-specific CD4+ T-cell recognition of LCLs expressing LANA antigens.
Shereen Sabbah et al. Blood 2012;119:2083-2092
©2012 by American Society of Hematology
LANA-specific CD4+ T cell recognition of PELs. (A) IFN-γ production by EYR- or GSP-specific T
cells incubated with VG-1 or JSC-1 PELs, respectively, fed either an enriched LANA protein or a
control protein preparation.
Shereen Sabbah et al. Blood 2012;119:2083-2092
©2012 by American Society of Hematology
vIRF3 and HLA class II expression by PELs and expression of CIITA and HLA class II after CIITA
transduction.
Shereen Sabbah et al. Blood 2012;119:2083-2092
©2012 by American Society of Hematology
LANA-specific CD4+ T-cell recognition of CIITA or control-transduced PELs. The HLA DQ7restricted GSP-specific clone was incubated in recognition assays with either nontransduced (-),
Cont-transduced, or CIITA-transduced (A) JSC-1 or (B) BC-1 PELs. VG-1–non...
Shereen Sabbah et al. Blood 2012;119:2083-2092
©2012 by American Society of Hematology
LANA-specific CD4+ T-cell recognition of CIITA-transduced JSC-1, BC-1, and VG-1 PELs or
unmanipulated BCBL-1 fed enriched LANA-protein.
Shereen Sabbah et al. Blood 2012;119:2083-2092
©2012 by American Society of Hematology