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Download and B9-TD - Blood Journal
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Ectopic expression of fibroblast growth factor receptor 3 promotes myeloma cell proliferation and prevents apoptosis by Elizabeth E. Plowright, Zhihua Li, P. Leif Bergsagel, Marta Chesi, Dwayne L. Barber, Donald R. Branch, Robert G. Hawley, and A. Keith Stewart Blood Volume 95(3):992-998 February 1, 2000 ©2000 by American Society of Hematology Expression of fibroblast growth factor receptor 3 (FGFR3) is achieved at variable levels in B9-WT and B9-TD cells.(A) Western blot analysis, probing with anti-FGFR3, shows the expression of FGFR3 in B9 clones: KMS11 myeloma cell line positive control for FG... Elizabeth E. Plowright et al. Blood 2000;95:992-998 ©2000 by American Society of Hematology Fibroblast growth factor receptor 3 (FGFR3) is functional in B9-WT and B9-TD Cells.Parental B9, B9 MINV, B9-WT, and B9-TD cells were subjected to an in vitro kinase assay with the use of antiFGFR3 to initially immunoprecipitate FGFR3. Elizabeth E. Plowright et al. Blood 2000;95:992-998 ©2000 by American Society of Hematology Cells expressing activated fibroblast growth factor receptor 3 (FGFR3) have an enhanced proliferative rate in the presence and absence of interleukin-6 (IL-6).Parental B9 (♦), B9-MINV (□), pooled B9-WT (▴), and B9-TD (×) cell lines were incubated in the pre... Elizabeth E. Plowright et al. Blood 2000;95:992-998 ©2000 by American Society of Hematology Ligand-stimulated cells expressing wild-type fibroblast growth factor receptor 3 (FGFR3) proliferate in the absence of interleukin-6 (IL-6).A chromogenic dye 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) assay was performed on IL-6–star... Elizabeth E. Plowright et al. Blood 2000;95:992-998 ©2000 by American Society of Hematology STAT3 phosphorylation in fibroblast growth factor receptor 3 (FGFR3)-expressing cells.FGFR3expressing and control cell lines were depleted of cytokine and then stimulated with or without interleukin-6 for 10 minutes at 37°C. Elizabeth E. Plowright et al. Blood 2000;95:992-998 ©2000 by American Society of Hematology STAT3 DNA binding is constitutively activated in B9-WT and B9-TD cells.Nuclear extracts were prepared from B9, B9 MINV, B9-WT#3, and B9-TD#14 stimulated with and without interleukin-6 for 10 minutes at 37°C. Elizabeth E. Plowright et al. Blood 2000;95:992-998 ©2000 by American Society of Hematology Elizabeth E. Plowright et al. Blood 2000;95:992-998 ©2000 by American Society of Hematology Elizabeth E. Plowright et al. Blood 2000;95:992-998 ©2000 by American Society of Hematology
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