Download • Karin et al, Proc. Natl. Acad. Sci. USA, Vol. 80, 1983, pages 4040

Title (en)
HUMAN METALLOTHIONEIN-II PROMOTER IN MAMMALIAN EXPRESSION SYSTEM
Publication
EP 0217822 B1 19930512 (EN)
Application
EP 86901613 A 19860211
Priority
• US 70129685 A 19850213
• US 80167485 A 19851125
Abstract (en)
[origin: WO8604920A1] An expression system for recombinant production of a desired protein comprises CHO cells transformed with a DNA
sequence having the desired protein coding sequence under control of the human metallothionein-II promoter. The cells can be maintained on
serum-free medium and induced in the presence of an induction mediator. In addition, the system may include an enhancer element and/or a
resistance-conferring gene to provide increased levels of expression. The system can process genomic as well as intronless DNA, and is capable of
producing proteins which have the same characteristics as those obtained from native sources. Human growth hormone which is indistinguishable
from that produced by the pituitary has been thus produced. Expression systems containing genes encoding alveolar surfactant protein (ASH),
apolipoproteins AI and AII (apoAI and apoAII), atrial natriuretic factor (ANF), erythropoetin (Epo), and renin are also contemplated.
Inventor
• KUSHNER, PETER, J.
• COFER, CLAIRE, L.
• FRIEDMAN, JEFFREY, S.
• TALMADGE, KAREN, D.
Applicant
SCIOS NOVA INC.
IPC 1-7 (main, further and additional classification)
C07K 13/00; C07K 15/08; C12N 5/00
IPC 8 full level (invention and additional information)
C07K 14/505 (2006.01); C07K 14/58 (2006.01); C07K 14/61 (2006.01); C07K 14/775 (2006.01); C07K 14/785 (2006.01); C12N 5/00 (2006.01); C12N 9/64 (2006.01); C12N 15/85 (2006.01)
CPC (invention and additional information)
C12N 9/6486 (2013.01); C07K 14/505 (2013.01); C07K 14/58 (2013.01); C07K 14/61 (2013.01); C07K 14/775 (2013.01); C07K 14/785 (2013.01);
C12N 15/85 (2013.01); C12Y 304/23015 (2013.01)
Citation (examination)
• Karin et al, Proc. Natl. Acad. Sci. USA, Vol. 80, 1983, pages 4040-4044.
• U.S., A, 4,446,235, Published 1 May 1984, Seeburg et al
Designated contracting state (EPC)
AT BE CH DE FR GB IT LI LU NL SE
EPO simple patent family
WO 8604920 A1 19860828; AT 89314 T 19930515; AU 5513586 A 19860910; AU 580145 B2 19890105; CA 1338829 C 19970107; DE 3688418
D1 19930617; DE 3688418 T2 19930826; DK 488386 A 19861215; DK 488386 D0 19861013; EP 0217822 A1 19870415; EP 0217822 A4
19890511; EP 0217822 B1 19930512; ES 551913 D0 19870801; ES 8707564 A1 19871016; US 5089397 A 19920218
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LAPSE BECAUSE OF EXPIRATION OF PROTECTION
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SCIOS NOVA INC.
1994-04-13 [RAP2] TRANSFER OF RIGHTS OF AN EP GRANTED PATENT
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1993-10-18 [NLV1] NL: LAPSED OR ANNULLED DUE TO FAILURE TO FULFILL THE REQUIREMENTS OF ART. 29P AND
29M OF THE PATENTS ACT
1993-06-18 [ET] FR: TRANSLATION FILED
1993-06-17 [REF] CORRESPONDS TO:
- Document : DE 3688418 19930617
1993-06-11 [ITF] IT: TRANSLATION FOR A EP PATENT FILED
- Owner name : ST. DR. CAVATTONI ING. A. RAIMONDI
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1990-10-24 [17Q] FIRST EXAMINATION REPORT
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