Download (Catalog # SL100432) 1. Description: Human ERBB2 kinase domain

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ERBB2 (kinase domain)
(Catalog # SL100432)
Lot: PKSP010
1. Description: Human ERBB2 kinase domain (amino acids Q679-V1255, GenBank entry
X03363), contains N-terminal GST fusion tags with thrombin cleavage sites
2. Product Information
Theoretical MW (ERBB2) 93.9 kDa (fusion protein)
Expression system: Baculovirus infected Sf9 cells
Purification: One-step affinity purification using glutathione agarose
Storage buffer: 50 mM Tris-HCl, pH 8.0, 100 mM NaCl, 5 mM DTT, 15 mM reduced
glutathione, 20% glycerol
Storage temperature: -80°C (avoid repeated freeze-thaw cycles !)
Shipping conditions: Dry ice
Protein concentration: 0.25 mg/ml (Bradford method using BSA as standard protein)
Method for determination of Km value & specific activity: Filter binding assay MAFC
membrane
Specific activity: 9.000 pmol/mg min
SignaGen® Laboratories
P.O. Box 661
Gaithersburg, MD 20884-0661
Fax: (301)-560-4919
Email: [email protected]
Website: www.signagenlabs.com
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