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where your research starts… ERBB2 (kinase domain) (Catalog # SL100432) Lot: PKSP010 1. Description: Human ERBB2 kinase domain (amino acids Q679-V1255, GenBank entry X03363), contains N-terminal GST fusion tags with thrombin cleavage sites 2. Product Information Theoretical MW (ERBB2) 93.9 kDa (fusion protein) Expression system: Baculovirus infected Sf9 cells Purification: One-step affinity purification using glutathione agarose Storage buffer: 50 mM Tris-HCl, pH 8.0, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol Storage temperature: -80°C (avoid repeated freeze-thaw cycles !) Shipping conditions: Dry ice Protein concentration: 0.25 mg/ml (Bradford method using BSA as standard protein) Method for determination of Km value & specific activity: Filter binding assay MAFC membrane Specific activity: 9.000 pmol/mg min SignaGen® Laboratories P.O. Box 661 Gaithersburg, MD 20884-0661 Fax: (301)-560-4919 Email: [email protected] Website: www.signagenlabs.com