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Association of CD26 with CD45RA outside lipid rafts attenuates cord blood T-cell activation by Seiji Kobayashi, Kei Ohnuma, Masahiko Uchiyama, Kouichi Iino, Satoshi Iwata, Nam H. Dang, and Chikao Morimoto Blood Volume 103(3):1002-1010 February 1, 2004 ©2004 by American Society of Hematology Cell surface expressions of CD3, CD26, CD28, CD45RA, and CD45RO on PBTCs and CBTCs. Data are represented by histograms in which cells were stained with stated mAbs (filled arrowheads) or isotype-matched mAbs (open arrowheads) (A) and by dot plots (B). Seiji Kobayashi et al. Blood 2004;103:1002-1010 ©2004 by American Society of Hematology Comitogenic effect of CD26 on CD3-dependent proliferation of CBTCs compared with PBTCs, CD45RA+ PBTCs, and CD45RO+ PBTCs. (A) Proliferation assays were performed as described in “Materials and methods” with PBTCs and CBTCs incubated in the presence of submi... Seiji Kobayashi et al. Blood 2004;103:1002-1010 ©2004 by American Society of Hematology Comodulation of CD26 with CD45RA in CBTCs. CD26, CD45RA, and CD45RO surface expression was determined by immunofluorescence as described in “Materials and methods.” PBTCs (A) and CBTCs (B) were analyzed for CD26, CD45RA, and CD45RO expression prior to and f... Seiji Kobayashi et al. Blood 2004;103:1002-1010 ©2004 by American Society of Hematology Attenuation of TCR/CD3 signal transduction following CD26 stimulation in CBTCs. (A) CD26activated protein tyrosine phosphorylation. Seiji Kobayashi et al. Blood 2004;103:1002-1010 ©2004 by American Society of Hematology CD26-mediated redistribution of signaling molecules. Seiji Kobayashi et al. Blood 2004;103:1002-1010 ©2004 by American Society of Hematology Colocalization of CD26 and CD45RA outside lipid rafts after cross-linking of CD26 in CTBCs. For staining CD45RO+ PBTCs, cells were incubated with biotinylated anti-CD26 mAb (1F7) and cross-linked with Texas red-conjugate streptavidin for patching, as descri... Seiji Kobayashi et al. Blood 2004;103:1002-1010 ©2004 by American Society of Hematology Association between CD26 and CD45RA in CBTCs. PBTCs (P) and CBTCs (C) were incubated with anti-CD26 mAb (1F7) and then cross-linked with secondary antibodies followed by crosslinking with the cleavable cross-linker DTSSP. CD26 and its cross-linked complex ... Seiji Kobayashi et al. Blood 2004;103:1002-1010 ©2004 by American Society of Hematology
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