Download NIH Project 1

DR. RAJ LAKSHMAN
PROFESSOR OF BIOCHEMISTRY,
MOLECULAR BIOLOGY & MEDICINE
CONTACT NUMBERS
TEL:(202)745-8330
FAX: (202)462-2006
EMAIL: [email protected]
DR. LAKSHMAN’S FUNDING
NIH GRANTS
Mechanisms of Actions of Alcohol & Omega3-Fatty Acids.
 To delineate the mechanisms of actions of alcohol and
omega3-fatty acids in altering the post-translational
modifications of glycoproteins and their functions.
Light & Heavy Alcohol, Paraoxonase & Oxidized LDL
Status.
 The major goal of this project is to evaluate the benefits
and deleterious effects of moderate and heavy drinking
on cardiovascular risk factors
Actions of Alcohol & Omega3-Fatty Acids
LIVER CELL
BLOOD COMPARTMENT
VLDL
LCAT
ST6 Gal 1 3’UTR
Binding Protein
(~41kD)
Cholesterol
uptake
Apo-E
CHE
HDL2
ST6 Gal 1 mRNA
Protein Complex
Free
CH
HDL3
UNSTABLE
ST6 Gal 1 mRNA
Asialoconjugates
Normal
ST6Gal1
X
Sialoconjugates
ω3-Fatty Acids
Cholesterol
Loaded
Macrophage
Bimodal Action of Alcohol on PON & CAD Status
LDL
Up-regulated
by light ethanol
HDL-PON
OX-LDL
Inactive
products
Plaque
Down-regulated
by heavy ethanol
Foam Cell
Macrophage