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SUPPLEMENTARY INFORMATION Supplementary Table S1: Primer sequences for real time PCR Gene 5´ primer 3´ primer Adcy9 GTGATGTCACCCTTGGACAC CCTGCAGCACTGAGCTGTT Akt1 CTCAGATGATCACCATCACG AGGAGAACTGGGGAAAGTGC Apoe CAGAGCTCCCAAGTCACACA TTCCTCCAGCTCCTTTTTGT App CACCTTGGTGATGCTGAAGA CTGCTGCATCTTGGAGAGG Bbs2 AAACCTGAAGGCGGTGAAC TCACCTGGTTCTTTGGCTTT Camk2b GCAGAATGTCCATTTCCACTG ACTCTGTCCGGTGAAACCAG Comt GACCGCTACCTTCCAGACAC GACGTTGTCAGCTAGGAGCAC Drd1a ATGCTGACTGGGCTGACTG CACCGCATCTCTCCAAATG Freq CCTCTACGACGGGCTGGTAT TGGCACAGGAAGAGGCTAGT Gria3 AGGCTACAACGTGTATGGAACA CCTCTCATTGCATGCTTCTG Grin2a CTTCACAGGCGTGTGCTCT AATGTGTACCCCATGGATGC Gsk3b CTGCTTCACCTCCTGCAAAC GGCGTTATTGGTCTGTCCAC Htr2a CACACTGGCTCAGTCACCAT GACAGCAGCCCAGGAACTTA Htr2c CATCGTTTGGGCAATATCAA TCACGAACACTTTGCTTTCG Impa2 GCTGCCACAGTCATCATCAG ACCACTCTGCACGACATGAG Maoa AGGAAAAGTCGCGAAGAAGG GGGTAATTTCAATGGCTGGA Maob TGGGAAGATTCCAGAGGATG TCTCTCCAGGAAGGTGTTGG Nedd4l GAGCAATGGGGTAGTCCTGA TTCAAAGGTTTCATACGGAGGT Npy TGAGAGAAAGCACAGAAAATGC TTTCATTTCCCATCACCACA Nsf CTTGGCCCTCATGAGAGAAG TCATCACCTTGGTCACTGTTG Prnp CCTTCTTAGCGGTACCAGTCC AGCAGCCAGTAGCCAAGGT Psen1 AGTGGGGACTGGAACACAAC AAAATGGCGAGCAGGAGTAA Reln ATGATCAGTGGGCTTTGGAC AGCCCATGTTGTCGTGAAA Rgs4 TTCATCTCTGTGCAGGCAAC AACATGTTCCGGCTTGTCTC Stead et al. Page 1 Rtn4 GCAAACAAGAGTGTTAAGGATGC TTTGGGGCTTTTTCAATCTG Slc1a2 CTATGCCGCACACAACTCTG TCCTCAACACTGCAGTCAGC Sybl1 ATGTGCGTGAAGAACGTCAA GCACAGTGGAGACACGATGA Syn2 ACTCCTGCCCTGTCTCCAC CCGGTTCCTTAAGTGTTCCA Synj1 GCCTTCAGACTCCTCACCAC GTTTGACACCACAAGCTCCA Tbp TCAAACCCAGAATTGTTCTCC GGGGTAGATGTTTTCAAATGC Tcf4 CGAATCACATGGGTCAGATG GCTGTTAAGGAAGTGGTCTCTTG Th TGTGTCCGAGAGCTTCAATG CGGGTCAAACTTCACAGAGA Xbp1 CATGGATTCTGACGCTGTTG ACAGGGTCCAACTTGTCCAG Supplementary Table S2: Complete gene expression data for all genes and all samples Table S2 contains log2-transformed expression values for all genes, with data presented individually from each GeneChip. Data was normalized by RMA (Irizarry et al., 2003) using a custom chip definition file based on Unigene build number 139 (January 2005). Column names indicate brain region, developmental age, and reference number of the biological replicate. Supplementary Table S3: Expression data for genes within groups generated by partitional clustering Table S3 lists all genes within each of the 26 clusters used in the analysis described for Figure 5. Mean expression data are included for each developmental stage and brain region. Stead et al. Page 2
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