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Shireen Saleque Ph.D
Affiliation:
City College, CUNY
Biology
"Cancer","Infectious and Immunological Diseases","Other"
Title: Assistant Professor
Rank: Assistant Professor
Address:
MR 716 160 Convent Avenue
New York, NY 10031
Contact:
Email: [email protected]
Telephone: 212-650-8538
Narrative
Our laboratory focusses on the elucidation of the molecular mechanisms of hematopoietic
development during various stages of organismal (embryonic versus adult) and cellular (stem,
progenitor, and effector) development in mammals. This includes determination of molecular players
and events, both genetic and epigenetic, regulating commitment, generation, differentiation and
function of multiple blood lineage cells. These investigations involve the murine model system and
also in vitro manipulation of primary and cultured (transformed) cells.
Recent work in the lab has illustrated novel regulatory mechanisms for fine tuning epigenetic activity
and modulating cellular differentiation during normal erythroid (red blood cell) and megakaryocytic
(platelet lineage) development. These processes are crucial for optimal gene expression during
normal development and could be potentially applicable for therapeutic intervention in blood cell
disorders and malignancies. Recently, the laboratory research has also diversified to explore
intersections between transcriptional regulation, chromatin function, signal transduction cascades
and cytoskeletal alterations in co-ordinating lineage specification and determination. These
processes will be examined in detail upon in vivo genetic ablation or manipulation of candidate
genes. In parallel new players and pathways involved in the above developmental systems and
processes will be identified by chromatin immmunoprecipitation (ChIPseq), expression profiling
(RNAseq) and protein-protein interaction (affinity purification and mass spectrometric analysis)
screens and the newly identified candidates characterized for their biochemical activities and
physiological functions.
Publications
1.
Sengupta A, Upadhyay G, Sen S, Saleque S. Reciprocal regulation of alternative PubMed
lineages by Rgs18 and its transcriptional repressor Gfi1b. J Cell Sci. 2016 Jan 01;
129(1):145-54.
2.
Upadhyay G, Chowdhury AH, Vaidyanathan B, Kim D, Saleque S. Antagonistic PubMed
actions of Rcor proteins regulate LSD1 activity and cellular differentiation. Proc Natl
Acad Sci U S A. 2014 Jun 03; 111(22):8071-6.
3.
Small C, Ramroop J, Otazo M, Huang LH, Saleque S, Govind S. An unexpected link PubMed
between notch signaling and ROS in restricting the differentiation of hematopoietic
progenitors in Drosophila. Genetics. 2014 Jun; 197(2):471-83.
4.
Chowdhury AH, Ramroop JR, Upadhyay G, Sengupta A, Andrzejczyk A, Saleque S. PubMed
Differential transcriptional regulation of meis1 by Gfi1b and its co-factors LSD1 and
CoREST. PLoS One. 2013; 8(1):e53666.
5.
Saleque S, Kim J, Rooke HM, Orkin SH. Epigenetic regulation of hematopoietic PubMed
differentiation by Gfi-1 and Gfi-1b is mediated by the cofactors CoREST and LSD1.
Mol Cell. 2007 Aug 17; 27(4):562-72.
6.
Zhou J, Saleque S, Ermakova O, Sepulveda MA, Yang Q, Eckhardt LA, Schildkraut CL, PubMed
Birshtein BK. Changes in replication, nuclear location, and expression of the Igh locus
after fusion of a pre-B cell line with a T cell line. J Immunol. 2005 Aug 15;
175(4):2317-20.
7.
Hock H, Hamblen MJ, Rooke HM, Schindler JW, Saleque S, Fujiwara Y, Orkin SH. Gfi-1 PubMed
restricts proliferation and preserves functional integrity of haematopoietic stem
cells. Nature. 2004 Oct 21; 431(7011):1002-7.
8.
Gordon SJ, Saleque S, Birshtein BK. Yin Yang 1 is a lipopolysaccharide-inducible PubMed
activator of the murine 3' Igh enhancer, hs3. J Immunol. 2003 Jun 01; 170(11):554957.
9.
Saleque S, Cameron S, Orkin SH. The zinc-finger proto-oncogene Gfi-1b is essential PubMed
for development of the erythroid and megakaryocytic lineages. Genes Dev. 2002 Feb
01; 16(3):301-6.
10. Saleque S, Singh M, Birshtein BK. Ig heavy chain expression and class switching in PubMed
vitro from an allele lacking the 3' enhancers DNase I-hypersensitive hs3A and hs1,2.
J Immunol. 1999 Mar 01; 162(5):2791-803.
11. Saleque S, Singh M, Little RD, Giannini SL, Michaelson JS, Birshtein BK. Dyad PubMed
symmetry within the mouse 3' IgH regulatory region includes two virtually identical
enhancers (C alpha3'E and hs3). J Immunol. 1997 May 15; 158(10):4780-7.
12. Birshtein BK, Chen C, Saleque S, Michaelson JS, Singh M, Little RD. Murine and PubMed
human 3'IgH regulatory sequences. Curr Top Microbiol Immunol. 1997; 224:73-80.
13. Shrivastava A, Saleque S, Kalpana GV, Artandi S, Goff SP, Calame K. Inhibition of PubMed
transcriptional regulator Yin-Yang-1 by association with c-Myc. Science. 1993 Dec
17; 262(5141):1889-92.
14. Riggs KJ, Saleque S, Wong KK, Merrell KT, Lee JS, Shi Y, Calame K. Yin-yang 1 PubMed
activates the c-myc promoter. Mol Cell Biol. 1993 Dec; 13(12):7487-95.
15. Saleque S, Ruiz N, Drickamer K. Expression and characterization of a carbohydrate- PubMed
binding fragment of rat aggrecan. Glycobiology. 1993 Apr; 3(2):185-90.
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