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VEGFR-3 and CD133 identify a population of CD34+ lymphatic/vascular endothelial precursor cells by Petri Salven, Satu Mustjoki, Riitta Alitalo, Kari Alitalo, and Shahin Rafii Blood Volume 101(1):168-172 January 1, 2003 ©2003 by American Society of Hematology VEGFR-3 cell-surface expression in human CD34+ cells.After immunomagnetic separation of CD34+ cells from human fetal liver, the CD34+ and CD34− cell fractions were stained with FITClabeled anti-CD34 and PE-labeled anti–VEGFR-3 mAbs and analyzed by 2-color ... Petri Salven et al. Blood 2003;101:168-172 ©2003 by American Society of Hematology RT-PCR for VEGFR-3 on human CD34+ and CD34− cells.CD34+ and CD34− cells derived from human fetal liver were analyzed for the expression of VEGFR-3 and GAPDH mRNAs. Petri Salven et al. Blood 2003;101:168-172 ©2003 by American Society of Hematology CD133, an early stem and precursor cell marker, is expressed on VEGFR-3+ cells.CD34+ cells derived from human fetal liver were analyzed for the expression of CD133 and VEGFR-3 (panel B); isotype control is shown in panel A. The results demonstrate that almo... Petri Salven et al. Blood 2003;101:168-172 ©2003 by American Society of Hematology Expansion of nonadherent VEGFR-3+ cells in liquid culture.A quantity of 1 × 105 freshly isolated CD34+cells from human fetal liver were incubated for 2 weeks in EC growth medium. Petri Salven et al. Blood 2003;101:168-172 ©2003 by American Society of Hematology Flow cytometric analysis of surface marker expression on VEGFR-3+ cells in isolated CD34+ cells, in monolayers generated from isolated CD34+ cells, and in HUVEC monolayers.Isolated cells coexpress VEGFR-3+ and stem/progenitor cell markers CD133 and CD34 (A). Petri Salven et al. Blood 2003;101:168-172 ©2003 by American Society of Hematology Expression of lymphatic EC markers LYVE-1 and podoplanin on VEGFR-3+ cells in monolayers generated from isolated CD34+ cells.Double immunostaining of EC monolayers derived from isolated fetal liver CD34+ cells detected adherent VEGFR-3+cells (blue; panels A... Petri Salven et al. Blood 2003;101:168-172 ©2003 by American Society of Hematology
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