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Edwin J. Squirewell
2nd
816
ST NW Apt 1, Rochester, MN 55901
[email protected]; 843-478-5974
LinkedIn: https://www.linkedin.com/pub/edwin-squirewell-ph-d/76/615/a01
RESEARCH EXPERIENCE
Mayo Clinic, Rochester, MN: Postdoctoral Research Fellow, 2014 – 2016
Role of Focal Adhesion Kinase and Cell Adhesion Remodeling in Melanoma
 Aseptic laboratory techniques.
 Mammalian cell culture and maintenance of multiple cell lines.
 Transfections using siRNA and lentivirus.
 Cell proliferation, migration, and invasion assays using live-cell imaging (IncuCyte
ZOOM).
 Cell/tissue section immunohistochemistry using automated instrumentation (BONDMAX).
 Confocal microscopy and flow cytometry.
 Utilized luciferase reporter assay in 96-well format to screen for human kinases involved
in the expression of osteopontin (Spp1), a protein overexpressed in melanoma that is
strongly associated with cancer invasion and metastasis.
 Bacterial cell culture, DNA cloning, PCR, qPCR, sequencing, and mutagenesis.
 DNA/RNA isolation from cells/tissues using QIAGEN kits and automated standard
protocols (QiaCube).
 Western blot
 Automated western blot (ProteinSimple Wes).
 Automated pipetting (epMotion).
 Maintained, genotyped, and bred genetically modified mouse colonies.
 Tumor xenograft implantation techniques (flank, intradermal, subcutaneous, intracardiac).
 Bioluminescence and fluorescence in vivo xenogen imaging (IVIS).
 Harvested tumor xenografts for cell culture.
 Mouse dissection and tissue collection.
 Performed injections and administered drugs in mice (gavage, intraperitoneal).
 Maintained inventory supply, performed routine maintenance on laboratory equipment,
trained and mentored new personnel.
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University of Iowa, Iowa City, IA: Graduate Research Fellow, 2009-2014
Interactions of Tamoxifen Metabolites with Human Cytosolic Sulfotransferase: Implications
for the Therapeutic Action and Toxicity of Tamoxifen
 Developed bacterial expression systems for recombinant protein production.
 Protein purification using ion exchange and affinity column chromatography.
 PCR, gel electrophoresis, sequencing.
 DNA isolation using QIAGEN kits.
 Protein analysis by SDS-PAGE and western blot.
 HPLC experience.
 Experience using radioactive materials (3H and 35S)
 Developed in vitro enzyme kinetic assays to determined mechanism of inhibition and
related kinetic constants (IC50, Vmax, Km, Kcat, Ki, product inhibition, alternate
substrates).
 UV spectroscopy (colorimetric enzyme assays).
 Synthetic organic chemistry techniques: thin layer chromatography, flash column
chromatography, rotary evaporation, lyophilization.
 Structure elucidation using proton NMR and LC-MS.
 Managed compressed gas and radiochemical inventory.
 Oversaw radiochemical disposal and radiation safety protocols.
 Oversaw annual radiochemistry audits.
Charleston Southern University, Charleston, SC: Undergraduate Research Fellow, 2007-2009
The Catalytic Mechanism of Recombinant & Native Sialyltransferase
 Conducted research with the goal of understanding the catalytic mechanism of mammalian
sialyltransferases.
 Utilized site-directed mutagenesis to generate mutant enzyme construct.
 Cloned, overexpressed, purified, and kinetically characterized recombinant proteins in
bacterial and insect cells.
Professional Skills
 Strong organizational, scientific, and computer skills (e.g., Microsoft Office, GraphPad
Prism, Sigma Plot, ChemDraw, Vector NTI).
 Extensive experience writing manuscripts and presenting research data.
 Effective problem solver with a keen eye for detail.
 Significant experience training and mentoring laboratory personnel.
 Able to work independently and as part of a team.
 Self-motivated, adaptable, able to learn new techniques and perform multiple tasks
simultaneously.
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EDUCATION
1. Ph.D. (2009 – 2014), University of Iowa
Pharmacy (Medicinal and Natural Products Chemistry)
2. B.S. (2005 – 2009), Charleston Southern University
Biochemistry
SELECTED PUBLICATIONS
1. Meves, A., Nikolova, E., Heim, B.J., Squirewell, E. J., Cappel, M.A., Pittelkow, M.R.,
Otley, C.C., Behrendt, N., Saunte, D.M., Andersen, J.L., Schenck, L.A., Weaver, A.L.,
Suman, V.J., (2015) “Tumor cell adhesion as a risk factor for sentinel lymph node
metastasis in primary cutaneous melanoma.” Journal of Clinical Oncology. 33(23): 25092515
2. Squirewell, E. J., and Duffel, M.W. (2015) “The Effects of Endoxifen and Other Major
Metabolites of Tamoxifen on the Sulfation of Estradiol Catalyzed by Human Cytosolic
Sulfotransferases hSULT1E1 and hSULT1A1*1.” Drug Metabolism and Disposition.
43(6): 843-850
3. Squirewell, E. J., Qin, X., and Duffel, M.W. (2014) “Endoxifen and Other Metabolites of
Tamoxifen Inhibit Human Hydroxysteroid Sulfotransferase 2A1 (hSULT2A1).” Drug
Metabolism and Disposition. 42(11): 1843-1850
4. Squirewell, E. J., "Interactions of Endoxifen and other major metabolites of Tamoxifen
with human sulfotransferases SULT2A1, SULT1E1, and SULT1A1*1: implications for
the therapeutic action and toxicity of Tamoxifen." PhD (Doctor of Philosophy) thesis,
University of Iowa, 2014. http://ir.uiowa.edu/etd/4761.
AWARDS AND RECOGNITION
2014
2013
2013
2012
2012
2009
2009
2009
2008
2005
Mayo Clinic Dermatology Research Fellowship
Trainee Member of the Holden Comprehensive Cancer Center in Cancer
Signaling and Experimental Therapeutics
FASEB/MARC Travel Award to Experimental Biology 2013
American Society for Pharmacology and Experimental Therapeutics (ASPET)
Joseph G. Cannon Excellence in Graduate Research Travel Award
University of Iowa Graduate Dean’s Fellowship Recipient
Charleston Southern University Chemistry Award
Beta Beta Beta National Biological Honor Society
South Carolina Independent Colleges and Universities Faculty/Student
Undergraduate Research Grant
South Carolina Physics Scholars Institute
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