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The Induction of Megakaryocyte Differentiation Is Accompanied by Selective Ser133 Phosphorylation of the Transcription Factor CREB in Both HEL Cell Line and Primary CD34+Cells by Giorgio Zauli, Davide Gibellini, Marco Vitale, Paola Secchiero, Claudio Celeghini, Alessandra Bassini, Sabina Pierpaoli, Marco Marchisio, Lia Guidotti, and Silvano Capitani Blood Volume 92(2):472-480 July 15, 1998 ©1998 by American Society of Hematology Western blot analysis of PSer133-CREB and total CREB protein performed in HEL cells stimulated for 30 minutes with increasing concentrations of TPO. Cells were treated with 0 (negative control, lane 1), 1 (lane 2), 10 (lane 3), 100 (lane 4), or 500 (lane 5)... Giorgio Zauli et al. Blood 1998;92:472-480 ©1998 by American Society of Hematology Western blot analysis of PSer133-CREB and total CREB protein performed in HEL stimulated with 100 ng/mL of TPO for various time points. Giorgio Zauli et al. Blood 1998;92:472-480 ©1998 by American Society of Hematology Western blot analysis of PSer133-CREB and total CREB protein performed in HEL stimulated with agonists inducing differentiation along either the megakaryocytic or erythroid lineages. Giorgio Zauli et al. Blood 1998;92:472-480 ©1998 by American Society of Hematology HEL cells were cultured in the presence of medium alone (negative control), TPO (100 ng/mL), Epo (2 U/mL), hemin (10−7 mol/L), forskolin (10−5 mol/L), or PMA (10−7 mol/L) for 30 minutes. Giorgio Zauli et al. Blood 1998;92:472-480 ©1998 by American Society of Hematology Giorgio Zauli et al. Blood 1998;92:472-480 ©1998 by American Society of Hematology Giorgio Zauli et al. Blood 1998;92:472-480 ©1998 by American Society of Hematology Western blot analysis of PSer133-CREB and total CREB protein, performed in HEL cells stimulated with 100 ng/mL TPO (lanes 1 through 4), 10−5 mol/L FK (lanes 5 and 6), 10−7 mol/L PMA (lanes 7 through 10), or left untreated (lane 11) in the absence (lanes 1, ... Giorgio Zauli et al. Blood 1998;92:472-480 ©1998 by American Society of Hematology Giorgio Zauli et al. Blood 1998;92:472-480 ©1998 by American Society of Hematology Giorgio Zauli et al. Blood 1998;92:472-480 ©1998 by American Society of Hematology Giorgio Zauli et al. Blood 1998;92:472-480 ©1998 by American Society of Hematology (A) Analysis of PSer133-CREB and αIIbβ3 by double (anti-PSer133-CREB polyclonal rabbit serum + anti-CD41a MoAb followed by GAR-FITC + GAM-TRIC) immunofluorescence in primary CD34+hematopoietic progenitor cells stimulated for various days with 100 ng/mL of T... Giorgio Zauli et al. Blood 1998;92:472-480 ©1998 by American Society of Hematology
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