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Intronic splicing of hyaluronan synthase 1 (HAS1): a
biologically relevant indicator of poor outcome in multiple
myeloma
by Sophia Adamia, Tony Reiman, Mary Crainie, Michael J. Mant, Andrew R. Belch,
and Linda M. Pilarski
Blood
Volume 105(12):4836-4844
June 15, 2005
©2005 by American Society of Hematology
HAS genes are expressed in MM. Representative GeneScan electropherograms for DNA
fragment analysis of HAS gene expression are shown.
Sophia Adamia et al. Blood 2005;105:4836-4844
©2005 by American Society of Hematology
MM B cells express aberrant splice variants of HAS1.
Sophia Adamia et al. Blood 2005;105:4836-4844
©2005 by American Society of Hematology
HAS1Vb expression by MM B cells correlates with poor survival.
Sophia Adamia et al. Blood 2005;105:4836-4844
©2005 by American Society of Hematology
Expression of HAS1 and novel variant genes and proteins in MM cell lines.
Sophia Adamia et al. Blood 2005;105:4836-4844
©2005 by American Society of Hematology
HAS1-expressing MM B cells synthesize a pericellular HA matrix.
Sophia Adamia et al. Blood 2005;105:4836-4844
©2005 by American Society of Hematology
Pericellular matrix synthesized by MM B cells includes HA: MM B cells express intracellular HA.
PEA in combination with indirect HA staining was used to verify the existence of HA molecules
in the pericellular matrix detected by PEA on Figure 2.
Sophia Adamia et al. Blood 2005;105:4836-4844
©2005 by American Society of Hematology