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26. KOLOKVIJ HRVATSKOG DRUŠTVA ZA TEORIJSKU I MATEMATIČKU BIOLOGIJU
Dimitar Kenanov
Friedrich Schiller University, Jena, Germany
Department of Bioinformatics
Metabolic Pathway Analysis and Elementary
Flux Modes
Elementary flux modes analysis is a method for Metabolic pathway analysis helping us
understanding the complex structure of the metabolic networks.
An elementary flux mode (EFM) can be described as a minimal set of enzymes which
can operate at steady state with all irreversible reactions used in the right direction
(Schuster et al., 1999). That means if an enzyme in an EFM is completely inhibited, then
the remaining enzymes of that EFM cannot operate on their own at steady state. This
concept is valuable for analysing enzyme deficiencies, which often lead to diseases, such as
favism, which is caused by the deficiency of glucose-6-phosphate dehydrogenase. As a
complete enzyme deficiency is comparable to the situation where a formerly active enzyme
is fully inhibited, it is interesting to see which EFMs of the complete system remain after
cancelling particular enzymes. This should allow one to decide which products are still
being synthesized and, thus, which enzyme deficiencies are harmful and which are not. The
concept of elementary flux modes is also useful in drug target identification, metabolic
reconstruction and finding the pathway with maximum yield of a certain product of
interest, so making it important for both biotechnology and medecine.
Četvrtak, 27. siječnja, 2005. u 15 sati
Seminar V krila Instituta Ruđer Bošković