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BY7201
BIOSEPARATION TECHNOLOGY
LTPC
3003
UNIT I
INTRODUCTION TO BIOSEPARATION
4h
Characterization of biomolecules and fermentation broth. Guidelines to recombinant protein
purification.
UNIT II
SOLID-LIQUID SEPARATION AND CELL DISRUPTION
6h
Solid liquid separation- microfiltration and centrifugation – theory and design for scaleup
operation. Cell disruption – Homogeniser, dynomill – principle, factors affecting disruption,
batch and continuous operation. Cell disruption by chemical methods.
UNIT III
CONCENTRATION AND PURIFICATION
7h
Liquid- liquid extraction – theory and practice with emphasis on aqueous two phase extraction.
Solid liquid extraction. Precipitation techniques using salt and solvent. Separation by
ultrafiltration, Dialysis, Electrophoresis.
UNIT IV
CHROMATOGRAPHY
15 h
Theory, practice and selection of media for – Gelfiltration chromatography, Ion exchange
chromatography, Hydrophobic interaction chromatography, reverse phase chromatography,
Affinity chromatography – Metal affinity chromatography, dye affinity chromatography,
immunosorbent affinity chromatography & Expanded bed chromatography. Scaleup criteria for
chromatography, calculation of no of theoretical plates and design.
UNIT V
FINAL POLISHING AND CASE STUDIES
13 h
Freeze drying, spray drying and crystallization. Purification of cephalosporin, aspartic acid,
Recombinant Streptokinase, Monoclonal antibodies, Tissue plasminogen activator, Taq
polymerase, Insulin.
TOTAL : 45 PERIODS
REFERENCES
1. Belter, P.A. et al., Bioseparations: Downstream Processing for Biotechnology, John-Wiley,
1988.
2. Janson J.C, & Ryden L. Protein Purification: Principles, High Resolution Methods and
Applications, VCH Pub., 1989.
3. Scopes R.K. – Protein Purification – Principles and Practice, Narosa, 1994.
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DOC/LP/01/28.02.02
LESSON PLAN
LP- BY7201
Sub Code & Name: BY7201 & Bioseparation Technology
Unit: I
UNIT I
Branch: M.Tech Biotechnology
Date: 31/12/2014
Page 1 of 6
INTRODUCTION TO BIOSEPARATION
Characterization of biomolecules and fermentation broth.
purification.
Session
No.
Semester: II
LP Rev. No: 00
Topics to be covered
Guidelines to recombinant protein
Time
(min)
1
Introduction to bioseparation
50
2
Characterization of biomolecules
50
3
Characterization of fermentation broth
50
4
Guidelines to recombinant protein
purification
50
4h
Ref. Book
(Page No.)
R1 (1-9)
R2 (1-12)
R1 (1-9)
R2 (1-12)
R3 (277-280)
R1 (1-9)
R2 (1-12)
R3 (277-280)
R2 (214-239)
Teaching
Method
LCD
LCD
LCD
LCD
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DOC/LP/01/28.02.02
LESSON PLAN
LP- BY7201
Sub Code & Name: BY7201 & Bioseparation Technology
Unit: II
UNIT II
Branch: M.Tech Biotechnology
Semester: II
LP Rev. No: 00
Date: 31/12/2014
Page 2 of 6
SOLID-LIQUID SEPARATION AND CELL DISRUPTION
6h
Solid liquid separation- microfiltration and centrifugation – theory and design for scaleup operation.
Cell disruption – Homogeniser, dynomill – principle, factors affecting disruption, batch and
continuous operation. Cell disruption by chemical methods.
Session
No.
Topics to be covered
Time
(min)
Ref. Book
(Page No.)
R1 (13-45)
R2 (100-118)
R3 (281-287)
R1 (47-75)
R2 (42-53)
R3 (287-292)
Teaching
Method
5
Solid liquid separationmicrofiltration
50
6
Solid liquid separationcentrifugation
50
7
Theory and design for scaleup
operation
50
R1 (47-75)
R2 (42-53)
LCD
8
Cell disruption – Homogeniser,
dynomill – principle, factors affecting
disruption
50
R1 (77-95)
R2 (13-25)
R3 (292-296)
LCD
9
Batch and continuous operation
50
10
Cell disruption by chemical methods
50
R1 (77-95)
R2 (13-25)
R3 (292-296)
R1 (77-95)
R2 (13-25)
R3 (292-296)
LCD
LCD
LCD
LCD
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DOC/LP/01/28.02.02
LESSON PLAN
LP- BY7201
Sub Code & Name: BY7201 & Bioseparation Technology
Unit: III
UNIT III
Branch: M.Tech Biotechnology
Semester: II
LP Rev. No: 00
Date: 31/12/2014
Page 3 of 6
CONCENTRATION AND PURIFICATION
7h
Liquid- liquid extraction – theory and practice with emphasis on aqueous two phase extraction.
Solid liquid extraction. Precipitation techniques using salt and solvent. Separation by ultrafiltration,
Dialysis, Electrophoresis.
Session
No.
Topics to be covered
Time
(min)
11
Liquid- liquid extraction
50
12
Theory and practice with emphasis
on aqueous two phase extraction
50
13
Solid liquid extraction
50
14
Precipitation techniques using salt
50
15
Precipitation techniques solvent
50
16
Separation by ultrafiltration
50
17
Dialysis, Electrophoresis
50
Ref. Book
(Page No.)
R2 (67-89)
R3 (296-300)
R2 (67-89)
R3 (300-301)
R1 (99-140)
R1 (221-235)
R2 (119-142)
R3 (208-281)
R1 (221-235)
R2 (119-142)
R3 (208-281)
R1 (237-260)
R2 (112-114)
R1 (260-264)
R2 (116-117)
Teaching
Method
LCD
LCD
LCD
LCD
LCD
LCD
LCD
4/6
DOC/LP/01/28.02.02
LESSON PLAN
LP- BY7201
Sub Code & Name: BY7201 & Bioseparation Technology
Unit: IV
Branch: M.Tech Biotechnology
UNIT IV
Semester: II
LP Rev. No: 00
Date: 31/12/2014
Page 4 of 6
CHROMATOGRAPHY
15 h
Theory, practice and selection of media for – Gelfiltration chromatography, Ion exchange
chromatography, Hydrophobic interaction chromatography, reverse phase chromatography, Affinity
chromatography – Metal affinity chromatography, dye affinity chromatography, immunosorbent
affinity chromatography & Expanded bed chromatography. Scaleup criteria for chromatography,
calculation of no of theoretical plates and design.
Session
No.
Time
(min)
Ref. Book
(Page No.)
Teaching
Method
Theory, practice and selection of
media for – Gelfiltration
chromatography
50
R2 (175-187)
LCD
20
Ion exchange chromatography
50
R2 (188-199)
LCD
21
Hydrophobic interaction
chromatography
50
R2 (200-213)
LCD
22
Reverse phase chromatography
50
R2 (200-213)
LCD
23
Affinity chromatography
50
R2 (214-238)
LCD
24
Metal affinity chromatography
50
R2 (214-238)
LCD
25
Dye affinity chromatography
50
R2 (214-238)
LCD
26
Immunosorbent affinity
chromatography
50
R2 (214-238)
LCD
27
Expanded bed chromatography
50
18, 19
Topics to be covered
R2 (214-238)
LCD
Journal papers
28, 29
Scale up criteria for chromatography
50
R2 (214-238)
LCD
30, 31
Calculation of no of theoretical
plates and design
50
R2 (214-238)
LCD
Review of Unit – IV
50
-
-
32
5/6
DOC/LP/01/28.02.02
LESSON PLAN
LP- BY7201
Sub Code & Name: BY7201 & Bioseparation Technology
Unit: V
Branch: M.Tech Biotechnology
UNIT V
Semester: II
LP Rev. No: 00
Date: 31/12/2014
Page 5 of 6
FINAL POLISHING AND CASE STUDIES
13 h
Freeze drying, spray drying and crystallization. Purification of cephalosporin, aspartic acid,
Recombinant Streptokinase, Monoclonal antibodies, Tissue plasminogen activator, Taq polymerase,
Insulin.
Session
No.
Topics to be covered
Time
(min)
Ref. Book
(Page No.)
Teaching
Method
33, 34
Freeze drying
50
R1 (307-331)
LCD
35
Spray drying
50
R1 (307-331)
LCD
Crystallization
50
R2 (273-303)
LCD
38
Purification of cephalosporin
50
Journal papers
LCD
39
Purification of aspartic acid
50
Journal papers
LCD
40
Purification of Recombinant
Streptokinase
50
R4 (270-277)
Journal papers
LCD
41
Purification of Monoclonal
antibodies
50
R4 (279-282)
Journal papers
LCD
42
Purification of Tissue plasminogen
activator
50
Journal papers
LCD
43
Purification of Taq polymerase
50
Journal papers
LCD
44
Purification of Insulin
50
Journal papers
LCD
45
Review of Unit - V
50
-
-
36, 37
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DOC/LP/01/28.02.02
LESSON PLAN
LP- BY7201
Sub Code & Name: BY7201 & Bioseparation Technology
Branch: M.Tech Biotechnology
Semester: II
LP Rev. No: 00
Date: 31/12/2014
Page 6 of 6
COURSE DELIVERY PLAN
WEEK
1
2
3
4
5
6
UNIT
I
I, II
II
II, III
III
III, IV
7
8
9
10
IV
11
12
IV, V
13
14
V
REFERENCE BOOKS
4. Belter, P.A., Cussler, E.L. and Hu, W.S., Bioseparations: Downstream Processing for
Biotechnology, John-Wiley, 2011.
5. Sivasankar, B., Bioseparations: Principles and Techniques, Prentice-Hall, 2005.
6. Stanbury, P.F., Whitaker, A. and Stephen J.H., Principles of Fermentation Technology,
Aditya Books, 1997.
7. Scopes R.K. – Protein Purification – Principles and Practice, Narosa, 1994.
8. Janson J.C, & Ryden L. Protein Purification: Principles, High Resolution Methods and
Applications, VCH Pub., 1989.
Prepared by
Approved by
Signature
Name
Dr. E. Nakkeeran
Prof. Sulochana Somasundaram
Designation
Associate Professor
Professor & Head
Department
Department of Biotechnology
Department of Biotechnology
Date
31.12.2014
31.12.2014
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