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table 2. The inhibition zone diameter methodological analysis of the effect of daily mouthwash (Class A) on the growth
of gram-positive bacteria
Inhibition Zone Diameter (mm) a
Microorganism
Control
ddH20
Control
Pure
Methanol
Undiluted
Disinfectant
Antiseptic
DF
1/2
DF
1/4
DF
1/8
DF
1/16
DF
1/32
Novobiocin
(30 g)
Class A – Daily Mouthwash §
Oral-B Pro-Expert Mouthwash (Disinfectant)
Active ingredients –Alcohol, Propyl paraben, and Poloxamer 407.
Gram-Positive Bacteria
Bacillus subtilis
Enterococcus Group
D
(E. faecalis)
Staphylococcus
aureus
Streptococcus Group
A
(GAS; S. pyogenes)
Streptococcus Group
B
(GBS; S. agalactiae)






16.00
 0.45
24.00
 0.58
18.66
 0.38
14.00
 0.33
3.33
 1.92


NI
17.00
 0.58
NI
26.33
 1.35
18.67
 0.19
16.00
 0.33
14.33
 0.19
12.00
 0.33
13.00
 0.88
36.00
 1.25



30.00
 1.57
11.67
 1.26


0.00
 0.00
NI *
NI
NI
NI
NI
NI
NI
NI
11.67
 0.51
11.33
 1.07
11.00
 1.15
22.67
 1.01
18.67
 0.51
17.33
 1.02
value ± SEM, n = 3 (the zone of inhibition [mm] including disk of 5 mm in diameter).
* NI = No Inhibition; TI = Total Inhibition (the zone of inhibition [mm] including disk of 5 mm in diameter is > 50 mm); DF = Dilution Factor.
§ Commercial brands are disclosed in accordance with ethical and propriety issues.
a Mean
Niveen M. Masri et al. The Immunomodulatory, Antimicrobial and Bactericidal Efficacy of Commonly Used Commercial
Household Disinfectants, Sterilizers and Antiseptics in Vitro: Putative Anti-Inflammatory Infection Control Mechanisms
and Comparative Biochemical Analysis of the Microbial Growth of Gram-Positive Bacteria. American Journal of Medical
and Biological Research, 2013, Vol. 1, No. 4, 103-133. doi:10.12691/ajmbr-1-4-4
© The Author(s) 2013. Published by Science and Education Publishing.