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Wolfson Centre for Inherited Neuromuscular Disease ===============CIND=============== Product Datasheet for Monoclonal Antibody Name: MANDOP32 - MOUSE ANTI- D2-Like Dopamine Receptors Clone No. 3F1 Specificity: D2-Like Dopamine Receptors Species: Human, rat Isotype: IgG2a Western blot: Stains 50 kD band in Rat brain total protein extract. Dilution: 1/30 50 kD Dopamine receptors Rat brain extract Ntera-2 cells Immunolocalization: Stains perinuclear endosomal granules in Ntera-2 cell cytoplasm and in rat brain neurons (receptors on the plasma membrane are also detected [not shown]) Immunogen: D3 N-terminal (a.a.2-10) Epitope: Amino-acids ASLSQLSSH Storage recommendation: Store one month's use at 4oC and remainder in aliquots at –20oC. Stable for at least 12 months under these conditions Supplied as liquid culture supernatant in DMEM/10% horse serum (contains AZIDE: toxic!) (Identical culture medium can be supplied as negative control) Reference: Endosomal location of dopamine receptors in neuronal cell cytoplasm. Elizabeth C. wolstencroft, Goran Simic, Nguyen Thi Man, Ian Holt, Le Thanh Lam, Paul R. Buckland, Glenn E. Morris. J Mol Hist(2007) 38: 333-340