Survey
* Your assessment is very important for improving the work of artificial intelligence, which forms the content of this project
Download Genetic Engineering
Document related concepts
no text concepts found
Transcript
Genetic Engineering • Clip • http://www.bbc.co.uk/programmes/b00plcl4# clips Steps • • • • • Isolating the gene of interest Building a cloning construct [Gene targeting] Transformation Selection Step 1: Culturing the gene of interest • Animation • Ecoli • Heat shock Eukaryotic vs. Plasmid DNA Restriction Enzymes • Endonucleases • Creating “sticky ends” • Like puzzle pieces Cloning Vector • Restriction enzyme sites as part of a multiple cloning site • Resistance marker or antibiotic resistance marker • Promoter Cloning Methods • Plasmid • Bacteriophage Bioethics Concerns • The Future of Food • Monsanto • Patents DIY Bio and Biobricks Agrobacterium tumefaciens • Agrobacterium • Gene gun • Horizontal gene transfer Three Vehicles E. coli DNA construction chassis Arabidopsis thaliana Agrobacterium tumefaciens DNA transfer chassis Applications in Research: Fluorescence • Tracking experiments • Expression studies Brainbow Knock-out and Knock-in Libraries molecular gastronomy the sweet science brazzein protein is 2000X sweeter than sugar miraculin is a flavor inverter Golden Rice Vitamin A deficiency • http://goldenrice.org/Content3Why/why1_vad.html • http://ucbiotech.org/resources/presentations /new_talks/shakespear/shakespeare2.html • http://www.scidev.net/en/opinions/goldengm-rice-shines-again.html Genetically Engineered Viruses • http://web.mit.edu/newsoffice/2009/virusbattery-0402.html • RNAi? • PCR and extracting genes • Lac operon
Related documents