Download Table 1 Hypothetical proteins in rat brain and their putative functions

Table 1 Hypothetical proteins in rat brain and their putative functions
Accession
Number
Protein name
Putative function by
sequence similarity
(Conserved domain)e)
Matches
(pMism
BLAST
Alignment
)
(identity to, %)
Theoretical/Observed
pI
Mr (kDa)
Metabolism-related
Q8BWM5
Similar to glycerol-3phosphate dehydrogenase
Q8IY16
Similar to RIKEN cDNA
2900024O10 gene
Q8WX76
BA113O24.2
Q91VA7
Tumor-related protein
a)
Q91VD9
Q91WP2a)
Q921G7
Q96IF0
Hypothetical 79.7 kDa
protein
Hypothetical 116.4 kDa
protein
RIKEN cDNA
0610010I20 gene
Aldehyde dehydrogenase
4 family, member A1
Glycerol-3-phosphate
dehydrogenase
(KOG2711)
Metallo-beta-lactamase
superfamily in
metabolism
(KOG0813)
Oxidoreductase
(KOG2450)
Oxidoreductase
(KOG0784)
Electron transporter or
NADH dehydrogenase
(ubiquinone)
(KOG2282)
Oxidoreductase
(KOG0450)
Oxidoreductase and
electron transport
(KOG2415)
Aldehyde
dehydrogenase
(KOG2455)
5
(9.43)
NH
6.46 / 8.23 34.6 / 37.2
6
(9.25)
NH
6.42 / 7.85 31.3 / 28.5
5
(7.91)
7
(10.59)
5
(9.19)
P30837
(94 %)
O77784
(88 %)
Q8BM16
(99 %)
6.54 / 7.75 57.2 / 53.5
9
(14.69)
6
(9.06)
Q80Y57
(96 %)
Q8BK82
(99 %)
6.36 /
116.4 /
7.25~7.55
115.6
7.34 /.8.05 68.1 / 63.8
9
(13.55)
P30038
(99 %)
8.25./ 8.21 61.7 / 61.5
8.76 / 8.30 42.2 / 41.5
5.51 /
5.45~5.85
79.7 / 79.7
2610008O03Rik protein
Q9D0K2a)
Coenzyme A transferase
(KOG3822)
6
(12.34)
P55809
(87 %)
8.73 / 8.33 56.0 / 55.5
Microtubule-associated
and GTP binding protein
(KOG0447)
8
(9.41)
O60313
(91 %)
7.18 / 7.30
Cytoskeleton
(KOG3527)
5
(7.95)
O43491
(91 %)
8.18 / 7.35 71.6 / 93.2
Cell motility and
structure (KOG0676)
5
(7.52)
P42025
(98 %)
5.98 / 7.25 42.3 / 47.0
Polymer formation
(KOG1376)
6
(9.66)
NH
4.87 / 5.80 37.2 / 59.9
Molecular chaperone
(KOG0101)
Chaperone-like function
(KOG0742)
6
(9.28)
5
(8.15)
O43301
(94 %)
NH
6.32 / 7.27 74.9 / 75.1
Cytoskeleton-related
Dynamin-like 120 kda
BAC26331 protein, mitochondrial
precursor (large GTP
binding protein) (largeg),
full insert sequence
Q8N4C4 Similar to erythrocyte
membrane protein band
4.1-like 2
Q8R5C5 Similar to ARP1 actinrelated protein 1 homolog
B, centractin beta
Similar to tubulin alpha 2
b)
Q8WU19
113.4 /
89.5
Chaperone-like protein
Q8K0U4
Q925I1
Similar to KIAA0417
gene product
TOB3
9.32 / 7.92
66.7 / 100
2
Table 1 (Continued)
Cell division or differentiation-related
Q8N959
Q9JJM9
Hypothetical protein
FLJ38330
CDCrel-1A
Cellular differentiation
(KOG2931)
Cell division and GTP
binding (KOG2655)
5
(9.53)
7
(9.63)
NH
5.89 / 6.90 35.2 / 48.2
Q99719
(98 %)
6.34 /
7.15~7.45
43.9 /
41.5~45.5
Q03154
(85 %)
P46460
(94 %)
5.89 / 7.17 45.8 / 45.3
Proteolysis-related
Q99JW2
RIKEN cDNA
1110014J22 gene
N-ethylmaleimide
sensitive factor
Proteolysis and
7
peptidolysis (KOG2275)
(8.68)
ATP-dependent peptidase
9
(KOG0741)
(13.22)
O60376c)
P1.11659_4
Integral membrane
protein (KOG2620)
Q8BH59
Solute carrier family 25
Q8BIX2
HSGCN1 homolog
Q9DBM1
1300003A17Rik protein
Q9NW31
Hypothetical protein
FLJ10348
Mitochondrial aspartate
or glutamate carrier
(KOG0751)
Intracellular transport
processes or DNA
binding (KOG1242 and
KOG2171)
Putative RNA-binding
proteins (KOG2138)
Multi-protein complexes
(PCI domain for
Q9QUL6
6.55 /
7.50~8.10
82.7 / 76.5
Miscellaneous
6
11.14
()
8
(13.67)
NH
6.40 / 6.85
38.7 / 39.8
O75746
(94 %)
8.43 / 8.72
74.6 /
68.5~72.2
7
(8.49)
Q9UE60
(91 %)
6.01 / 7.65
115.4 /
115.8
5
(7.55)
5
(7.93)
NH
6.53 / 8.51
Q9Y677
(99 %)
5.57 / 7.71
103.0 /
215.8
45.0 / 10.5
signaling) (KOG1497)
Q9Y512
Protein CGI-51
Cell surface protein
(KOG2602)
8
(11.26)
NH
6.44 / 7.85
52.0 / 52.5
6
(11.71)
P49411
(89 %)
7.26 /
7.77~7.88
49.9 / 46.7
8
(12.65)
NH
6.67 /
7.37~8.02
49.6 / 49.5
8
(13.40)
6
(10.17)
7
(12.80)
NH
7.14 / 8.17
41.8 / 41.3
NH
6.00 / 5.45
25.6 / 88.9
NH
8.83 /
8.50~8.70
28.3 / 29.2
Unknown
CAD34734b,
Q9CXS1
Homo sapiens sequence Unknown
42 from patent
wo0222660
Homo sapiens sequence Unknown
33 from patent
wo0218424
5730568A12Rik protein Unknown
Q9D9F6
1700082C19Rik protein Unknown
Q9DA45
1700021B03Rik protein Unknown
d)
CAD33306
Rat proteins were separated by 2-DE and identified by MALDI-MS, following in-gel digestion with trypsin. The spots representing the identified
proteins are indicated in Fig 1 and are designated with their accession numbers of SWISS-PROT or the other database (www.ncbi.nlm.gov). The
theoretical/observed molecular weight (MW) and pI values, as well as the number of matching peptides (matches) and the probability of
assignment (pMism) are given. Some hypothetical proteins were detected in mouse hippocampusa) (Unpublished data, Shin et al., 2003), human
mesothelial cell lineb), human lymphocytec) or human bronchial cell lined) (Unpublished data).
e)
Accession number of Conserved Domain Database (CDD, www.ncbi.nlm.nih.gov). The sequences of hypothetical proteins were submitted to
BLAST and putative conserved domains were obtained by sequence similarity. NH, there were no comparable homologs with known function.