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ANTIBODIES AND ANIMAL RED BLOOD CELLS Antibodies and Animal Red Blood Cells Glutaraldehyde Stabilized RBC's Fresh Red Blood Cells Red Blood Cell Antibodies www.cedarlanelabs.com/RBC CEDARLANE® is an ISO 9001 and ISO 13485 registered company Stabilized Glutaraldehyde Stabilized Red Blood Cells Glutaraldehyde stabilized animal red blood cells are produced from freshly washed animal RBCs by brief exposure to a glutaraldehyde-buffer solution and exhaustive washing in saline. This treatment largely preserves the native antigenicity of the erythrocytes while rendering the cells generally resistant to lysis by osmotic shock, freeze-thawing or immune hemolysis. These reagents are used directly in hemagglutination procedures, or may be coupled with various proteins for hemagglutination testing. The glutaraldehyde stabilized RBCs are available as cell suspensions in saline with 0.1% sodium azide. Tanned sheep red blood cell reagents are produced by coupling purified human serum proteins to glutaraldehyde stabilized sheep RBCs with tannic acid. The sensitized stabilized sheep RBCs are useful reagents in hemagglutination testing of antibodies and in the hemagglutination inhibition testing of other serum proteins. These reagents are supplied as cell suspensions in saline with 0.1% sodium azide. Specificity Glutaraldehyde Stabilized Chicken RBCs (10%) Glutaraldehyde Stabilized Guinea Pig RBCs (10%) Glutaraldehyde Stabilized Mouse RBCs (1%) Glutaraldehyde Stabilized Rabbit RBCs (10%) Glutaraldehyde Stabilized Rat RBCs (1%) Glutaraldehyde Stabilized Sheep RBCs (10%) Glutaraldehyde Stabilized Bovine RBCs (10%) Glutaraldehyde Stabilized Dog RBCs (10%) Glutaraldehyde Stabilized Horse RBCs (10%) Glutaraldehyde Stabilized Turkey RBCs (10%) Glutaraldehyde Stabilized Cat RBCs (10%) Glutaraldehyde Stabilized Goat RBCs (10%) Glutaraldehyde Stabilized Hamster RBCs (10%) Glutaraldehyde Stabilized Pig RBCs (10%) Size 20 mL 15 mL 5 x 2 mL 20 mL 5 x 2 mL 20 mL 20 mL 10 mL 20 mL 20 mL 5 mL 20 mL 5 mL 20 mL Cat. # CLD88 CLD18 CLD38 CLD48 CLD58 CLD68 CLD78 CLD98 CLD108 CLD118 CLD128 CLD138 CLD148 CLD168 Price $ (USD) 181 181 181 181 181 181 181 181 181 181 181 181 181 181 Size 10 mL 10 mL 10 mL Cat. # CLD68LT CLD68LS-1 CLD68LS-2 Price $ (USD) 323 386 386 Tanned Sheep Red Blood Cells Specificity Glutaraldehyde Stabilized, Tanned Sheep RBCs (10%), Lyophilized Human IgG Sensitized, Glutaraldehyde Stabilized Sheep RBCs (1%), Lyophilized Human Albumin Sensitized, Glutaraldehyde Stabilized Sheep RBCs (1%), Lyophilized Fresh Fresh Red Blood Cells in Alsever’s Washed, fresh red blood cells from sheep, rat, mouse, and guinea pig are available as stabilized 10% suspensions in Alsever’s solution. The whole blood is aseptically collected and prepared to order from our colony of animals. These washed cell suspensions are useful for the titration of complement, adsorption procedures, testing for agglutinins, and for the preparation of stroma as particulate reagents. Specificity Guinea Pig Red Blood Cell Suspension (10%) Mouse Red Blood Cell Suspension (10%) Rat Red Blood Cell Suspension (10%) Sheep Red Blood Cell Suspension (10%) Chicken Red Blood Cell Suspension (10%) 2 Size 25 mL 10 mL 25 mL 100 mL 25 mL Cat. # CLC1800 CLC3800 CLC5800 CLC6800 CLC8800 Price $ (USD) 260 208 233 344 354 www.cedarlanelabs.com – ANTIBODIES AND ANIMAL RED BLOOD CELLS FROM CEDARLANE Antibodies to Red Blood Cells Antibodies Anti-Red Blood Cell sera are high titer rabbit antisera prepared by immunization with washed intact red blood cells of the species listed. These antisera are useful in a variety of assays including hemagglutination and complement fixation, and to identify lymphocyte subsets. These antisera are supplied as frozen products containing 0.05% sodium azide. IgG fractions of high titer antisera are purified by salt fractionation and ion exchange chromatography. The IgG fractions are tested against their immunogens, analyzed for immunoglobulin purity and protein concentration and sterile filtered. The IgG fractions are sold as sterile frozen products in phosphate buffered saline, pH 7.8. IgM fractions of high titer antisera are purified by salt fractionation, ion exchange chromatography and gel filtration procedures. The IgM antibodies are tested against their immunogens, analyzed for immunoglobulin purity and protein concentration and sterile filtered. The IgM fractions are sold as sterile frozen products in phosphate buffered saline, pH 7.8. Fluorochrome conjugated IgG products are prepared by the covalent coupling of Fluorescein or Texas Red to high titer IgG antibodies. The conjugates are purified to remove free fluorochrome, under-conjugated and over-conjugated antibodies. The fluorescent products are analyzed for their fluorochrome : protein ratios; they will exhibit bright fluorescence on the red blood cells of suitable species. The fluorochrome conjugated IgG products are sold as frozen reagents in phosphate buffered saline, pH 7.8 and contain 0.05% sodium azide. Specificity Format Isotype Species Reactivity Size Cat. # Bovine Red Blood Cells Antiserum IgG Fraction IgM Fraction FITC Texas Red Antiserum IgG Fraction IgM Fraction FITC Texas Red Antiserum IgG Fraction IgM Fraction FITC Texas Red Antiserum IgG Fraction IgM Fraction FITC Texas Red Antiserum IgG Fraction IgM Fraction FITC Texas Red Antiserum IgG Fraction IgM Fraction FITC Texas Red Antiserum IgG Fraction IgM Fraction FITC Texas Red Rabbit serum Rabbit IgG Rabbit IgM Rabbit IgG Rabbit IgG Rabbit serum Rabbit IgG Rabbit IgM Rabbit IgG Rabbit IgG Rabbit serum Rabbit IgG Rabbit IgM Rabbit IgG Rabbit IgG Rabbit serum Rabbit IgG Rabbit IgM Rabbit IgG Rabbit IgG Rabbit serum Rabbit IgG Rabbit IgM Rabbit IgG Rabbit IgG Rabbit serum Rabbit IgG Rabbit IgM Rabbit IgG Rabbit IgG Rabbit serum Rabbit IgG Rabbit IgM Rabbit IgG Rabbit IgG Bovine Bovine Bovine Bovine Bovine Cat Cat Cat Cat Cat Chicken Chicken Chicken Chicken Chicken Dog Dog Dog Dog Dog Goat Goat Goat Goat Goat Guinea Pig Guinea Pig Guinea Pig Guinea Pig Guinea Pig Hamster Hamster Hamster Hamster Hamster 2mL 20mg 5mg 3mg 3mg 2mL 20mg 5mg 3mg 3mg 2mL 20mg 5mg 3mg 3mg 2mL 20mg 5mg 3mg 3mg 2mL 20mg 5mg 3mg 3mg 2mL 20mg 5mg 3mg 3mg 2mL 20mg 5mg 3mg 3mg CLA7840 CLAG7840 CLAM7840 CLFAG7840 CLRAG7840 CLA12840 CLAG12840 CLAM12840 CLFAG12840 CLRAG12840 CLA8840 CLAG8840 CLAM8840 CLFAG8840 CLRAG8840 CLA9840 CLAG9840 CLAM9840 CLFAG9840 CLRAG9840 CLA13840 CLAG13840 CLAM13840 CLFAG13840 CLRAG13840 CLA1840 CLAG1840 CLAM1840 CLFAG1840 CLRAG1840 CLA14840 CLAG14840 CLAM14840 CLFAG14840 CLRAG14840 Cat Red Blood Cells Chicken Red Blood Cells Dog Red Blood Cells Goat Red Blood Cells Guinea Pig Red Blood Cells Hamster Red Blood Cells Price $ (USD) 168 229 417 281 428 168 229 417 281 428 168 229 417 281 428 168 229 417 281 428 168 229 417 281 428 168 229 417 281 428 168 229 417 281 428 continued on next page ANTIBODIES AND ANIMAL RED BLOOD CELLS FROM CEDARLANE – www.cedarlanelabs.com 3 Specificity Format Isotype Species Reactivity Size Cat. # Horse Red Blood Cells Antiserum IgG Fraction IgM Fraction FITC Antiserum IgG Fraction IgM Fraction FITC Texas Red Purified FITC Purifed Biotin FITC PE APC Antiserum IgG Fraction IgM Fraction FITC Antiserum IgG Fraction IgM Fraction FITC Texas Red Purified Biotin FITC PE Antiserum IgG Fraction IgM Fraction FITC Texas Red Antiserum IgG Fraction IgM Fraction FITC Antiserum IgG Fraction IgM Fraction FITC Texas Red Antiserum IgG Fraction IgM Fraction FITC Antiserum IgG Fraction IgM Fraction FITC Rabbit serum Rabbit IgG Rabbit IgM Rabbit IgG Rabbit serum Rabbit IgG Rabbit IgM Rabbit IgG Rabbit IgG Rabbit IgG Rabbit IgG Rat IgG2b Rat IgG2b Rat IgG2b Rat IgG2b Rat IgG2b Rabbit serum Rabbit IgG Rabbit IgM Rabbit IgG Rabbit serum Rabbit IgG Rabbit IgM Rabbit IgG Rabbit IgG Rat IgG2b Rat IgG2b Rat IgG2b Rat IgG2b Guinea Pig serum Guinea Pig IgG Guinea Pig IgM Guinea Pig IgG Guinea Pig IgG Rabbit serum Rabbit IgG Rabbit IgM Rabbit IgG Rabbit serum Rabbit IgG Rabbit IgM Rabbit IgG Rabbit IgG Rabbit serum Rabbit IgG Rabbit IgM Rabbit IgG Rabbit serum Rabbit IgG Rabbit IgM Rabbit IgG Horse Horse Horse Horse Human Human Human Human Human Human Human Human Human Human Human Human Monkey Monkey Monkey Monkey Mouse Mouse Mouse Mouse Mouse Mouse Mouse Mouse Mouse Rat Rat Rat Rat Rat Rat Rat Rat Rat Sheep Sheep Sheep Sheep Sheep Swine Swine Swine Swine Turkey Turkey Turkey Turkey 2mL 20mg 5mg 3mg 2mL 20mg 5mg 3mg 3mg 250 μg 100 μg 200 μg 100 μg 100 μg 50 μg 100 μg 2mL 20mg 5mg 3mg 2mL 20mg 5mg 3mg 3mg 250 μg 100 μg 100 μg 50 μg 2mL 20mg 5mg 3mg 3mg 2mL 20mg 5mg 3mg 2mL 20mg 5mg 3mg 3mg 2mL 20mg 5mg 3mg 2mL 20mg 5mg 3mg CLA10840 CLAG10840 CLAM10840 CLFAG10840 CLA2840 CLAG2840 CLAM2840 CLFAG2840 CLRAG2840 CL8020AP CL8020F CL7607AP CL7607B CL7607F CL7607PE CL7607APC CLA15840 CLAG15840 CLAM15840 CLFAG15840 CLA3840 CLAG3840 CLAM3840 CLFAG3840 CLRAG3840 CL8926AP CL8926B CL8926F CL8926PE CLA5810 CLAG5810 CLAM5810 CLFAG5810 CLRAG5810 CLA5840 CLAG5840 CLAM5840 CLFAG5840 CLA6840 CLAG6840 CLAM6840 CLFAG6840 CLRAG6840 CLA16840 CLAG16840 CLAM16840 CLFAG16840 CLA11840 CLAG11840 CLAM11840 CLFAG11840 Human Red Blood Cells Human Glycophorin A (CD235a) Human Glycophorin A (CD235a) (clone: YTH 89.1) Monkey Red Blood Cells Mouse Red Blood Cells Mouse Erythroid Cells (Ly-76) (clone: TER-119) Rat Red Blood Cells Sheep Red Blood Cells Swine Red Blood Cells Turkey Red Blood Cells Please contact CEDARLANE for more information or visit our website: In U.S.A.: 1210 Turrentine Street Burlington, NC 27215 Toll Free: 1-800-721-1644 ph: (336) 513-5135 fax: (336) 513-5138 E-mail: [email protected] Pricing is listed in U.S. dollars and is subject to change without notice. In CANADA: 4410 Paletta Court Burlington, ON L7L 5R2 Toll Free: 1-800-268-5058 ph: (289) 288-0001 fax: (289) 288-0020 E-mail: [email protected] Price $ (USD) 168 229 417 281 168 229 417 281 428 204 204 246 169 180 144 283 168 229 417 281 168 229 417 280 428 141 81 92 93 168 229 417 281 428 168 229 417 280 168 229 417 281 428 168 229 417 280 168 229 417 281