Download Nivedita Priya

Nivedita Priya
Mobile: 09582574411 ~ E-Mail: [email protected]
Synopsis
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Ph.D in Medical Biochemistry from Vallabhbhai Patel Chest Institute, University of Delhi in
2012.
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Research work pursued under the guidance of Dr. H.G. Raj, Professor & Head, (Formerly) Dept. of
Biochemistry, V. P Chest Institute, University of Delhi.
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Worked as a Senior Scientific Officer, Medical Biochemistry in ADEP Clinical Research and
Diagnostics (ACRD), New Delhi.
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Worked with Dr. Amit Sharma in Malaria Lab on Mammalian cell Culture, International Centre for
Genetic Engineering and Biotechnology (ICGEB) as Research Assistant.
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Accredited with two prestigious research fellowships from CSIR and DSIR.
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Proficient in the field of platelet aggregation studies and Thrombotic research.
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Excellent understanding of the subject with strong motivations to pursue active research in Drug
Discovery.
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Hardworking, diligent, resourceful and a keen observer with strong communication and analytical
skills. Expertise in paperwork and documentation.
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Knowledge of Quality related documentation like preparation of SOP, ISO 9001: 2009, NABL 15189.
Key Skill Set
Mammalian & Bacterial Cell Culture Handling ~ ELISA ~ Blotting (Western) ~ Protein Purification ~
Affinity Chromatography ~ GPC AKTA ~ Ion Exchange Chromatography ~Drug Screening ~ Thrombotic
Studies Enzymatic Chemi-Luminisence~Polyacrelamide Gel Electrophoresis ~ Agarose Gel
Electrophoresis ~ SpectroflourometeryFlow Cytometry – Fluorimetery ~ Plasmid isolation- Animal
Handling ~ Transfections
JOB Details
Senior Scientific officer, Medical Biochemistry
May 2014- June 2015
ADEP Clinical Research and Diagnostics, B-1 Ext/A-39 Mohan coop. Industrial estate,
Mathura road, New Delhi-110044.
Title:
Period:
Organization:
Description:
Responsibilities:
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The Company is into diagnostics and Research. It comprises of four established Depts.
Biochemistry
Serology
Immunology
Microbiology
The Scientific officer is responsible to the lab Director of ACRD
Provide educational programs for the medical and laboratory staff. Conducting monthly
examinations, Supervision of technical operations.
Giving dissertation training and conducting clinical studies on the “occurrence of UTI in
Diabetic patients”.
Plan, set goals, develop and allocate resources appropriate to the medical laboratory.
Ensure that there are sufficient qualified personnel with adequate documented training and
experience meet the needs of the laboratory
Relate and function effectively with customers.
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Provide resources and training opportunities for laboratory staff to facilitate testing activities
in a safe work environment consistent with test requirements and personnel capabilities.
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Ensure all complaints, requests or suggestions are taken care appropriately
Ensure good staff morale
Ph.D Details
Title:
Period:
Guide:
Institute.
Description:
“Studies on the Development of Anti-platelet Candidate Drug”.
Jul’07 – Mar’12.
Dr. H.G. Raj – Professor and Head, Department of Biochemistry, Vallabhbhai Patel Chest
Responsibilities:
Platelet aggregation studies invitro and invivo,
LPS induced prothrombotic studies for the
factors like iNOS, eNOS, Cox-1, Cox-2, Thromboxane, cGMP, and Ca+2 levels, in various cells
like lymphomonocytes, aortic endothelium, platelets etc and FeCl 3 induced thrombus
formation which resulted into the discovery of a potential Antiplatelet agent.
Result:
Indian Patent is obtained for two lead molecules and the next step of finding a
suitable industrial partner is underway.
Published Works:
Kindly refer to annexure.
Various compounds synthesized by the Dept. of Chemistry (University of Delhi) were
screened for their anti platelet activities in Dept. of Biochemistry, VPCI. The short listed
molecules were further studied in rats for their action mechanism.
Work Experience
Malaria Lab, International Centre for Genetic Engineering and Biotechnology, New Delhi.
As Research Assistant under guidance of Dr. Amit Sharma from Oct’06 – Mar’07.
Research Topic:
Techniques Used:
Highlights:
Purification of Sporozoite Trap-A (Thrombospondin Related Anonymous Protein)
in Mammalian Expression System.
Transfection in HEK-293T cells (mammalian cell line), Protein Purification by Affinity Column
Chromatography, Gel Permeation Chromatography etc, Western blotting and developing on
the film by using ECL method (Enhanced Chemiluminescence), Bacterial cell culturing and
Plasmid isolation.
TRAP A protein bulk production for crystallization and to understand the structure by X Ray
Crystallography.
Trainings
Title:
Acetoxy Drug-Protein- Transacetylase Catalyzed Activation of Platelets Nitric Oxide Synthase by
Polyphenolic Peracetates.
Organization: Vallabhbhai Patel Chest Institute, Department of Biochemistry, University of Delhi, Delhi.
Duration:
Jan’06 – Jun’06.
Title:
Enzymes & various biomarkers in detection of liver and biliary tract diseases.
Organization: Chandan Diagnostic Centre and Blood Bank, Faizabad.
Duration:
Jun’05 – July’05.
Title:
Distillery and Water Effluent Treatment.
Organization: Mohan Mekin’s Private Limited, Lucknow.
Duration:
Apr’05 – May’05.
Symposia/ Workshops Attended
Event:
Topic:
Venue:
7th Indo-Italian Workshop on Chemistry and Biology of Antioxidants in Nov’10.
Characterization of No Aspirin Properties of 7, 8, Di Acetoxy-4-Methyl Coumarin.
Department of Chemistry, University of Delhi, Delhi.
Event:
Topic:
Venue:
Indo-Italian Workshop on Chemistry and Biology of Antioxidants in Dec’09.
Poster Presentation.
Department of Chemistry, University of Delhi, Delhi.
Event:
Topic:
Venue:
International Conference on Advances in Free Radical Research in Mar’09.
Natural Products, Antioxidants and Radio Protectors.
C.S.M. Medical University & Era’s Lucknow Medical College.
Event:
Topic:
Venue:
National Symposium in Jan’09.
Emerging Trends in Nitric Oxide Research: Impact on Health, Disease and Drug Development.
Vallabhbhai Patel Chest Institute, University of Delhi, Delhi.
Event:
Topic:
Venue:
Symposium in Sep’08.
Clinical Genomics, a satellite meeting of HGM 2008.
Institute of Genomic & Integrative Biology (IGIB).
Event:
Topic:
Venue:
IFI seminar in Sep’08.
Influenza Viruses in various Lung Diseases.
Vallabhbhai Patel Chest Institute, University of Delhi, Delhi.
Event:
Topic:
Venue:
National Seminar in Apr’08.
Environmental Lung Diseases National Seminar.
Vallabhbhai Patel Chest Institute, University of Delhi, Delhi.
Event:
Topic:
Venue:
Workshop in Sep’07.
Digital Flow Cytometry: Application in Cell Biology and Immunology.
Institute of Nuclear Medicine and Allied Sciences (INMAS).
Academia
 Ph.D in Biochemistry from Vallabhbhai Patel Chest Institute, Delhi in 2012.
 M.Sc. in Biochemistry from Allahabad Agricultural Institute (Deemed University), Allahabad in 2006. 87%
marks.
 B.Sc. (Biology) from Saket Degree College, Dr. R.M.L. Avadh University, Faizabad in 2004. 65% marks.
 12th from Canossa Convent Girl’s Inter College from U.P. Board in 2000. 69% marks.
 10th from Canossa Convent Girl’s Inter College from U.P. Board in 1998. 76% marks
Highlights
 Received Centre for Scientific and Industrial Research - Senior Research Fellowship Award in 2010.
 Received Department of Scientific and Industrial Research - Junior Research Fellowship Award in 2007
for 2 years.
Personal Dossier
Correspondence Address:
Permanent Address:
Linguistic Skills:
Reference:
H.No. 977, Sector 37, Faridabad-121003, Haryana.
H. No. 3/327, Gomti Nagar, Lucknow – 226010, U.P.
English and Hindi.
 Mr. Riju R Nair, ADEP Clinical Research and Diagnostics, B-1 Ext/A-39 Mohan coop. Industrial estate,
Mathura road, New Delhi-110044.
o Ph No. 9811072668.
 Dr. H.G. Raj - Professor/ Head, Department of Biotechnology, Vallabhbhai Patel Chest Institute, Delhi.
o Mobile: 099738179162, E-Mail: [email protected].
 Dr. V.S. Parmar – Head, Department of Chemistry, University of Delhi, Delhi.
o Mobile: 09818528933, E-Mail: [email protected].
 Dr. Ashok K. Prasad - Associate Professor, Department of Chemistry, University of Delhi, Delhi.
o Mobile: 09818131666. E-Mail: [email protected].
 Dr. Sunil K. Sharma - Associate Professor, Department of Chemistry, University of Delhi, Delhi.
o Mobile: 09818915790, E-Mail: [email protected].
ANNEXURE
S.No Name of the
Journal
Month and Title of Research paper
Year of the
publication
Single or with Co Author(s)
1.
Journal
of May, 2011
Pharmacy and
Pharmacology
Characterization of a
Unique
Dihydropyrimidinone,
Ethyl 4(4‘Heptanoyloxyphenyl)6-Methyl-3,4
Dihydropyrimidin-2-One5-Carboxylate, as an
Effective Antithrombotic
Agent in Experimental
Model of Rats.
Nivedita Priya, Prabhjot Singh, Sumati
Bhatia, Bikash Medhi, Ashok K. Prasad,Virinder S.
Parmar, Hanumantharao G. Raj.
2.
Bioorganic and
Medicinal
Chemistry
June 2010
Characterization of 4Methyl-2-Oxo-1, 2Dihydroquinolin-6-yl
Acetate as an Effective
Antiplatelet Agent.
Nivedita Priya, Anjali Gupta, Karam
Chand, Prabhjot Singh, Abha
Kathuria, Hanumantharao G. Raj, Virinder S.
Parmar, Sunil K. Sharma.
3
Bioorganic and
Medicinal
Chemistry
Feb 2012
Substrate Specificity of
Coumarins and
Quinolones towards
Calreticulin Mediated
Transacetylation:
Investigations on
Antiplatelet Function.
AbhaKathuria, NiveditaPriya, Karam
Chand, PrabhjotSingh, Anjali
Gupta, SarahjJalal, ShilpiGupta, Hanumantharao
G. Raj, Sunil K. Sharma
4
Biochemie
Sep 2010
Specificity of Calreticulin
Transacetylase to Acetoxy
Derivatives of
Benzofurans: Effect on the
Activation of Platelet
Nitric Oxide Synthase.
AnjaliGupta, NiveditaPriya, Sarah
Jalal, PrabhjotSingh, KaramChand, Hanumanthar
ao G. Raj, Virinder S. Parmar, Anthony L.
DePass, Sunil K. Sharma.
5.
Bioorganic and
Medicinal
Chemistry
Feb 2009
Specificities of Calreticulin
Transacetylase to Acetoxy
Derivatives of 3-Alkyl-4Methylcoumarins: Effect
on the Activation of Nitric
Oxide Synthase.
AbhaKathuria, Anjali Gupta, Nivedita Priya,
PrabhjotSingh, Hanumantharao G. Raj, Ashok K.
Prasad,Virinder S. Parmar, Sunil K. Sharma.
6.
Protein &
Peptide Letters
May 2011
Protein acyltransferase
function of purified
calreticulin: the exclusive
role of P-domain in
mediating protein
acylation utilizing
acyloxycoumarins and
acetyl CoA as the acyl
group donors.
Prabhjot Singh, Prija Ponnan, Nivedita Priya,
Tapesh K. Tyagi, Marco Gaspari, Shibu Krishnan,
Giovanni Cuda, Paritosh Joshi, Jasvinder K.
Gambhir, Sunil K. Sharma, Ashok K. Prasad,
Luciano Saso, Ramesh C. Rastogi, Virinder S.
Parmar, Hanumantharao G. Raj
7.
Bioorganic
Chemistry
Feb 2012
Calreticulin
transacetylase: a novel
enzyme-mediated protein
acetylation by acetoxy
derivatives of 3-alkyl-4methylcoumarins.
Sarahj Jalal, Karam Chand, Abha Kathuria,
Prabhjot Singh, Nivedita Priya, B. Gupta,
Hanumantharao G. Raj, Sunil K. Sharma
8.
Journal
of May 2010
Biochemistry
Protein Acyltransferase
Function of Purified
Calreticulin. Part 1:
Characterization of
Propionylation of Protein
Utilizing Propoxycoumarin
as the Propionyl Group
Donor.
Prabhjot Singh, Prija Ponnan, Shibu Krishnan
Tapesh K. Tyagi, Nivedita Priya, Bansal S, Scumaci
D, Gaspari M, CudaG, Joshi P, Gambhir JK, Saluja
D, Prasad AK, Saso L, Rastogi RC,Parmar VS, Raj
HG.
9.
Biochemie
Calreticulin Transacylase:
Genesis, Mechanism of
Action and Biological
Applications.
Ranju Kumari, Seema Bansal, Garima Gupta,
Shvetambri Arora,Ajit Kumar, Sanjay Goel,
Prabhjot Singh, Prija Ponnan, Nivedita Priya,
Tapesh K. Tyagi et al.
10
Comprehensive Book
Bioactive
Chapter
Natural
Products,
Volume
7,
Chapter 4.
Sep 2010
Structural Modifications
and Drug Development,
Prospects for the
Development of
Polyphenolic Acetateas
the Potential Drug
Candidate: A Review.