Download Molecular Biology EU Exercise 4/ Seminar 4 Molecular mechanisms

Molecular Biology EU
Exercise 4/ Seminar 4
Molecular mechanisms of DNA replication and DNA repair mechanisms.
Mode of DNA synthesis (dispersive, semiconservative, conservative); Meselson-Stahl experiment,
DNA topoisomerase, the topological problem;
Prokaryotic DNA replication: enzymes involved in replication, oriC, DnaA, DnaB, DnaC, prepriming
complex, primosome, replisome, single-strand binding proteins (SSB), leading strand, lag strand,
Okazaki fragment, DNA polymerases and their exonuclease activity of DNA polymerases.
Eukaryotic DNA replication: enzymes involved in replication, autonomously replicating sequences
(ARS), origin recognition complex (ORC), initiation regions, proliferating cell nuclear antigen (PCNA),
replication factor C (RFC), replication protein A (RPA), replication factories, leading strand, lag strand,
Okazaki fragment, endonuclease FEN-1, RNase H; telomerase, TRF protein.
Regulation of eukaryotic genome replication: cell cycle, Cdc6p; replication licensing factors (RLF),
cyclins, cyclin-dependent kinases.
Mutation and mutagens: causes of mutations, effects of mutations.
DNA repair: categories of DNA repair system, enzymes involved in DNA repair.
Recommended reading:

“Molecular Biology of the Cell”, Alberts B. (e.g. Chapter 5. DNA Replication, Repair, and
Recombination)
Available online at: http://www.ncbi.nlm.nih.gov/books/NBK21054/?term=genomes)

„Genomes", Brown TA (e.g. Chapter 13 Genome Replication, Chapter 14, Mutation, Repair
and Recombination.)
Available online at: http://www.ncbi.nlm.nih.gov/books/NBK21128/?term=genomes

http://mol-biol4masters.masters.grkraj.org//Molecular_Biology_Table_Of_Contents.html