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Oxidant Stress Enhances Adenylyl Cyclase Activation by C.M. Tan, S. Xenoyannis, and R.D. Feldman Circulation Research Volume 77(4):710-717 October 1, 1995 Copyright © American Heart Association, Inc. All rights reserved. The effect of H2O2 on adenylyl cyclase activation. C.M. Tan et al. Circ Res. 1995;77:710-717 Copyright © American Heart Association, Inc. All rights reserved. Dose-dependent enhancement of adenylyl cyclase activity mediated by H2O2. C.M. Tan et al. Circ Res. 1995;77:710-717 Copyright © American Heart Association, Inc. All rights reserved. H2O2-mediated enhancement of adenylyl cyclase activity: time course. C.M. Tan et al. Circ Res. 1995;77:710-717 Copyright © American Heart Association, Inc. All rights reserved. The effect of deferoxamine on H2O2-mediated enhancement of adenylyl cyclase activity. C.M. Tan et al. Circ Res. 1995;77:710-717 Copyright © American Heart Association, Inc. All rights reserved. The effects of hydroxyl radical scavengers on H2O2-mediated enhancement of adenylyl cyclase activity. C.M. Tan et al. Circ Res. 1995;77:710-717 Copyright © American Heart Association, Inc. All rights reserved. The effect of xanthine oxidase/purine (XO/P) on adenylyl cyclase activity. C.M. Tan et al. Circ Res. 1995;77:710-717 Copyright © American Heart Association, Inc. All rights reserved. The effect of deferoxamine on xanthine oxidase/purine (XO/P)–mediated enhancement of adenylyl cyclase activity. C.M. Tan et al. Circ Res. 1995;77:710-717 Copyright © American Heart Association, Inc. All rights reserved. The effect of tyrosine kinase inhibition on H2O2-mediated enhancement of adenylyl cyclase activity. C.M. Tan et al. Circ Res. 1995;77:710-717 Copyright © American Heart Association, Inc. All rights reserved. The effect of PKC (C-kinase) inhibition on H2O2-mediated enhancement of adenylyl cyclase activity. C.M. Tan et al. Circ Res. 1995;77:710-717 Copyright © American Heart Association, Inc. All rights reserved. Representative autoradiograph of SDS-PAGE demonstrating PT-mediated ADP ribosylation of the guanine regulatory protein of inhibition (Giα) and/or Goα in A10 cells. + represents lanes with lysates incubated with [32P]NAD and PT; −, lanes with lysates incubated with [32P]NAD alone. C.M. Tan et al. Circ Res. 1995;77:710-717 Copyright © American Heart Association, Inc. All rights reserved. Representative autoradiograph of SDS-PAGE demonstrating CT-mediated ADP ribosylation of the guanine regulatory protein of stimulation (Gsα) in A10 cells. + represents lanes with lysates incubated with [32P]NAD and CT; −, lanes with lysates incubated with [32P]NAD alone. C.M. Tan et al. Circ Res. 1995;77:710-717 Copyright © American Heart Association, Inc. All rights reserved. Representative autoradiographs of SDS-PAGE demonstrating the effect of H2O2 on PT- and CTmediated ADP ribosylation of Giα/Goα and Gsα, respectively, in A10 cells. C.M. Tan et al. Circ Res. 1995;77:710-717 Copyright © American Heart Association, Inc. All rights reserved.