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Cat#: M1310-2 GAPDH Quantity: 100ul Product Type: Mouse mAb Isotype: IgG2b, λchain Clone ID: 1-G2 Applications: WB, ICC Species reactivity: Human, mouse, rat Lot#: See on the tube Positive control: Hela, NIH/3T3, A431, D3 Form: Liquid Subcellular location: Cytoplasm, nucleus MW(kDa): 36 Database links: SwissProt P04406(huamn) P16858(mouse) P04797( rat) Description: GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Immunogen: Synthetic peptide (KLH-coupled) Recommended Dilutions: WB: 1:2000-1:5,000 ICC: 1:200 Buffer: 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. Storage: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. .Purity: ProA affinity purified Fig 1: Western blot analysis on Hela (1) and NIH/3T3 (2) cell lysates using anti-GAPDH Mouse mAb. Fig2: ICC staining GAPDH in D3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). Fig3: ICC staining GAPDH in A431 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue). Background References: 1. "High-resolution structure of human D-glyceraldehyde-3-phosphate dehydrogenase."Jenkins J.L., Tanner J.J.Acta Crystallogr. D 62:290-301(2006) 2. "Structural analysis of human liver glyceraldehyde-3-phosphate dehydrogenase."Ismail S.A., Park H.W.Acta Crystallogr. D 61:1508-1513(2005)