Download Description: GAPDH (Glyceraldehyde-3

Cat#: M1310-2
GAPDH
Quantity: 100ul
Product Type: Mouse mAb
Isotype: IgG2b, λchain Clone ID: 1-G2
Applications: WB, ICC
Species reactivity: Human, mouse, rat
Lot#: See on the tube
Positive control: Hela, NIH/3T3, A431, D3
Form: Liquid
Subcellular location: Cytoplasm, nucleus
MW(kDa): 36
Database links: SwissProt P04406(huamn) P16858(mouse) P04797( rat)
Description: GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) has both glyceraldehyde-3-phosphate
dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. It
participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is a
key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate
(G3P) into 3-phospho-D-glyceroyl phosphate.
Immunogen: Synthetic peptide (KLH-coupled)
Recommended Dilutions:
WB: 1:2000-1:5,000
ICC: 1:200
Buffer: 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol.
Preservative: 0.05% Sodium Azide.
Storage: Store at +4°C after thawing. Aliquot store
at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
.Purity: ProA affinity purified
Fig 1: Western blot analysis on Hela (1)
and NIH/3T3 (2) cell lysates using
anti-GAPDH Mouse mAb.
Fig2: ICC staining GAPDH in D3 cells (red). Cells were
fixed in paraformaldehyde, permeabilised with 0.25%
Triton X100/PBS and counterstained with DAPI in order
to highlight the nucleus (blue).
Fig3: ICC staining GAPDH in A431 cells (red). Cells
were fixed in paraformaldehyde, permeabilised with
0.25% Triton X100/PBS and counterstained with DAPI
in order to highlight the nucleus (blue).
Background References:
1.
"High-resolution structure of human D-glyceraldehyde-3-phosphate dehydrogenase."Jenkins J.L., Tanner
J.J.Acta Crystallogr. D 62:290-301(2006)
2.
"Structural analysis of human liver glyceraldehyde-3-phosphate dehydrogenase."Ismail S.A., Park H.W.Acta
Crystallogr. D 61:1508-1513(2005)