Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
K-RAS exon 2 EGFR exon 19 conventional-PCR conventional-PCR ≅ 5% C>T ≅ 5% C>A ≅ 44% C>T ≅ 35% C>A TP53 exon 5 conventional-PCR ≅ 5% G>A ≅ 5% G>T antisense TT-fast-COLD-PCR TT-fast-COLD-PCR ≅ 58% C>T TP53 exon 6 TP53 exon 7 conventional-PCR conventional-PCR ≅ 5% G>T conventional-PCR ≅ 5% C>T antisense TT-fast-COLD-PCR EGFR exon 20 TT-fast-COLD-PCR ≅ 67% G>A ≅ 78% G>T TP53 exon 8 TP53 exon 9 conventional-PCR conventional-PCR ≅ 5% C>T ≅ 5% C>T ≅ 5% C>T antisense TT-fast-COLD-PCR ≅ 63% G>T TT-fast-COLD-PCR ≅ 33% C>T TT-fast-COLD-PCR ≅79% C>T TT-fast-COLD-PCR ≅82% C>T Supplemental Figure 2 (expanded version of Figure 2). Sanger sequencing following 50-plex fast-TT-COLD-PCR or, alternatively, conventional 50-plex PCR. A mixture of genomic DNA from several cell lines (~5% mutation abundance) was pre-amplified using a 50-plex PCR using gene specific primers containing common sequences on the 5’ end. The sample was then split in eight parallel tubes and fast-TT-COLD-PCR was then performed using a single primer approach to co-amplify all 50 amplicons. Eight mutated sequences within the 50 amplicons were evaluated for. These targets represent sequences for which mutation-containing DNA and appropriate primers were available.