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Supplementary Table I
Structural Statistics of CEP-1
Distance restraints
Total NOE
2183
Intra-residue
838
Inter-residue
Sequential (|i-j| = 1)
465
Medium-range (|i-j| < 5)
321
Long-range (|i-j| > 5)
445
Intermolecular (ambiguous)
114 (44 experimental determined)
Hydrogen bonds
40
Dihedral angle restraints













NOE Assignment Completeness
Backbone amide protons
93.8%
Sidechain protons
70.0%
R.M.S. deviation from experimental restraints
Distance restraints (Å)
0.035 ± 0.003
Dihedral angle restraints ()
1.22 ± 0.08
Deviations from idealized geometry
Bond lengths (Å)
Bond angles ()
Impropers ()
Averaged pairwise r.m.s.d. (Å)a
Backbone
Heavy
Number of conformers (out of)
0.004
0.559
0.461
OD+SAM
1.16 ± 0.49
1.52 ± 0.43
20 (100)
OD
0.52 ± 0.20
0.92 ± 0.19
Ramachandran plot (percentage of residues)b
Core region:
89.1 %
Allowed region:
8.1 %
Generous region:
2.4 %
Disallowed region:
0.5 %
a. Residues from 532-555 (OD) and 575-642 (SAM) were used for alignment.
b. Residues from 528-642 were used for the Ramachandran plot.
SAM
0.51 ± 0.11
1.10 ± 0.17
Supplementary Table II
Structural Statistics of Dmp53
Distance restraints
Total NOE
Intra-residue
Inter-residue
Sequential (|i-j| = 1)
Medium-range (|i-j| < 5)
Long-range (|i-j| > 5)
Intermolecular (ambiguous)
Hydrogen bonds
Dihedral angle restraints











NOE Assignment Completeness
Backbone amide protons
Sidechain protons
1519
561
268
211
235
240 (184 experimental determined)
46


96.9%
71.6%
R.M.S. deviation from experimental restraints
Distance restraints (Å)
0.055 ± 0.002
Dihedral angle restraints ()
1.221 ± 0.052
Deviations from idealized geometry
Bond lengths (Å)
Bond angles ()
Impropers ()
Averaged pairwise r.m.s.d. (Å)a
Backbone
Heavy
Number of conformers (out of)
0.007
0.655
0.522
0.47 ± 0.15
0.94 ± 0.18
20 (100)
Ramachandran plot (percentage of residues)b
Core region:
90.2 %
Allowed region:
9.6 %
Generous region:
0.2 %
Disallowed region:
0.0 %
a. Residues from 321-325, 330-335, 341-359, and 366-383 were used for alignment
b. Residues from 315-385 were used for the Ramachandran plot.