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Sample CV Defined for Research Emphasis BRUTUS BUCKEYE The Ohio State University Office of Health Sciences 1063 Graves Hall, Columbus OH 43210 [email protected] (555) 555-5555 EDUCATION Indiana University Bloomington, IN Ph.D. Biophysical Chemistry May 20XX Advisor: XXX XXXX, Ph.D. Thesis: "Unfolding of Thymidylate Synthase. Studies of Wild Type Enzyme and Engineered Disulfide Mutants" University of California San Francisco M.S. Biophysical Chemistry B. S. Chemistry (Cum Laude) San Francisco, CA February 20XX May 20XX RESEARCH EXPERIENCE Research Interests Altering the Substrate Specificity of Thymidylate Synthase Protein Inhibitors from Bacteriophages Peptide Antibiotics Research Lab Work Experience The Ohio State University Postdoctoral Fellow, Oncology Division Columbus, OH March 20XX-Present Study the yeast model for cellular iron metabolism Conduct experiments contributing to the understanding of mitochondria in iron related diseases Cloned SSC2 (SSQ1), a mitochondrial heat shock protein, using classical S. cerevisiae molecular genetic techniques Show involvement of Ssc2p in Yfh1p (frataxin) maturation to functional form Meet on a weekly basis with interdisciplinary team to discuss project progress Brutus Buckeye, page 2 University of California San Francisco Research Assistant San Francisco, CA June 200X-December 200X Designed and studied several site-specific mutants of Thymidylate Synthase having altered substrate specificity Catalyzed mutants methylation of deoxycytidine monophosphate with high specificity and catalytic efficiency. Constructed synthetic genes for two small protein inhibitors from bacteriophages namely, Uracil Glycosylase Inhibitor from phage PBS2 and RNA Polymerase Inhibitor from T7 phage Expressed, purified and characterized proteins Technical Skills Protein Biochemistry: Purification, Electrophoresis, and Enzyme Kinetics Spectroscopy: UV/Visible, Fluorescence, Circular Dichroism, and NMR Molecular Biology: General molecular biology procedures including PCR, cloning, mutagenesis Research Grant Department of Defense Yeast Model for Cellular Iron Metabolism Cancer Research Program Postdoctoral Traineeship Award Grant (DAMD XX-XXX-X-XXXX, Modification XXXXXX), March 200X– April 200X Research Awards Best Ph.D. Thesis in Biophysical Chemistry, Kofi Annan Memorial Prize, June 200X American Chemical Society Massachusetts Section Scholastic Achievement Award, March 200X PUBLICATIONS The 23S ribosomal RNA m5U1939 methyltransferase, RumA, is a 4Fe-4S protein. Buckeye, B, Annan K Manuscript in preparation 2.2 Å crystal structure of RumA, a RNA m5U methyltransferase. Hume D, Buckeye, B Annan K Manuscript in preparation Characterization of the 23S rRNA m5U1939 methyltransferase from Escherichia coli. Buckeye, B, Mendeleyev I, Annan K Biochemistry (2000) 277 8835-8840 Brutus Buckeye, page 3 A novel dCMP methylase by engineering thymidylate synthase. Buckeye, B Annan K, Mendeleyev I Biochemistry (19XX) 36 15909-17 Covalent reinforcement of a fragile region in the dimeric enzyme thymidylate synthase stabilizes the protein against chaotrope-induced unfolding. Ford H, Buckeye, B, Mendeleyev I, Galileo P Biochemistry (19XX) 35 7150-8 Thermal stabilization of thymidylate synthase by engineering two disulfide bridges across the dimer interface. Ford H, Buckeye, B, Mendeleyev I, Galileo P Biochem Biophys Res Commun (19XX) 235 89-94 Conformation of the flexible bent helix of Leu1-zervamicin in crystal C and a possible gating action for ion passage. Buckeye, B, Galileo P Biopolymers (19XX) 34 721-35 CONFERENCES AND PRESENTATIONS The Fourth BioPathways Consortium Meeting, Intelligent Systems for Molecular Biology, Edmonton, Alberta, Canada, August 20XX Name of Talk Title Here Physiological Genomics of Cardiovascular Disease: from Technology to Physiology San Francisco, California, February 20XX Name of Talk Title Here Bay Area Bioinformatics Discussion GroupStanford, California, January 200X Name of Talk Title Here NIH-NHLBI Programs for Genomic Applications, External Scientific Panel Review Bethesda, Maryland, June 200X Name of Talk Title Here Iconix Pharmaceuticals, Mountain View, California, June 200X Name of Talk Title Here Brutus Buckeye, page 4 Department of Neurosciences, University of New Mexico Health Sciences Center Albuquerque, New Mexico, October 200X Name of Talk Title Here National Center for Genome Resources , Santa Fe, New Mexico, October 200X Name of Talk Title Here Bay Area RNA Club ,San Francisco, California, June 19XX Name of Talk Title Here TEACHING EXPERIENCE Teaching Interests Cell and molecular biology, Bioinformatics and genomics RNA and protein structure and function Scientific writing and speaking Gender and science Ethical issues in biotechnology Teaching Experience Xavier University Instructor, Biology Department Cincinnati, OH Autumn 200X Taught lecture and lab to 20 upper-division biology majors Developed lab exercise based on thesis research where students cloned different mutations in 16S rRNA into an E. coli expression vector and analyzed the growth phenotype of the mutant cells Created bioinformatics lab exercise based on tools publicly available on the web Evaluated highly by students for stimulating independent thinking and for demonstrating student interest Brutus Buckeye, page 5 INDUSTRY EXPERIENCE Scientific Chemicals Research Assistant Los Angeles, CA January- September 200X Assisted in the manufacturing of amino acid derivatives and unusual amino acids for peptide syntheses Optimized and scaled up syntheses of several derivatives Participated in research lab developing teamwork and consulting skills Ensure costumers satisfaction internationally in peptide/amino acid manufacturers in India, U.S. and Europe such as Advanced Chemtech, Anaspec and Senn Chemicals UNIVERSITY SERVICE The Ohio State University Association for Students in Science Columbus, OH June 200X- Present Provide leadership as chair of the Programs Committee Organize and lead monthly chapter meetings attended by 50-75 people Invite guest speakers to ensure students’ exposure to career development topics The Ohio State University Health Sciences Seminar Columbus, OH Autumn 200X Greeted students to seminar communicating program content Conducted exit interviews to evaluate success of seminar ADDITIONAL SKILLS Foreign Language, Proficient in Spanish, oral, writing and reading Computer Skills, Proficient in Microsoft Office HONORS AND AWARDS Lydia Jones Library Prize, Indiana University, 200X Thom Prize in Chemistry, University of California San Francisco, 19XX Phi Beta Kappa, Inducted January, 19XX Senior Service Award, University of California San Francisco, 19XX Brutus Buckeye, page 6 PROFESSIONAL AFFILIATIONS American Association for the Advancement of Science 19XX–present American Physiological Society 19XX- present Brutus Buckeye, page 7 REFERENCES XXX XXXX, Ph.D Associate Professor Department of Chemistry The Ohio State University 123 Gravel Hall Columbus, OH 43210 555-555-5550 [email protected] XXXXXXXXX XXX, Ph.D. Associate Professor Department of Chemistry Indiana University 456 Shoe Center College Park, ID 20815 555-555-5551 [email protected] X.X. XXXXX, M.D. Associate Investigator University of California, San Francisco P.O. Box XXX San Francisco, CA 94100-9100 555-555-5552 [email protected] XXXXXX XXXXX, Ph.D. Associate Professor Gladstone Institutes University of California, San Francisco 789 Parnassus Avenue San Francisco, CA 94143-0130 555-555-5553 [email protected]
