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Chemokine SDF-1 enhances circulating CD34+ cell
proliferation in synergy with cytokines: possible role in
progenitor survival
by Jean-Jacques Lataillade, Denis Clay, Catherine Dupuy, Sylvain Rigal, Claude
Jasmin, Philippe Bourin, and Marie-Caroline Le Bousse-Kerdilès
Blood
Volume 95(3):756-768
February 1, 2000
©2000 by American Society of Hematology
CXCR-4 expression is heterogeneous on PB and BM CD34+ cells and is up-regulated on PB
Inc+CD34+ cells.BM and PB CD34+ cells were purified immediately after density gradient
separation (Inc−, plots a and c) or after incubation on a plastic support (Inc+, plo...
Jean-Jacques Lataillade et al. Blood 2000;95:756-768
©2000 by American Society of Hematology
CXCR-4 surface overexpression on PB Inc+CD34+ cells is correlated with a decrease in the
CXCR-4 intracellular pool and protein synthesis.PB CD34+ cells were purified (A) without (Inc−)
or (B) with (Inc+) overnight incubation on a plastic support.
Jean-Jacques Lataillade et al. Blood 2000;95:756-768
©2000 by American Society of Hematology
PB Inc+CD34+CXCR-4high cells have a primitive progenitor phenotype.PB CD34+ cells were
purified after incubation on a plastic support (Inc+), stained for CD34-PerCP, CD38-FITC, CD71PE, c-Kit-PE, or Thy-1-PE and CXCR-4-biotin-APC and analyzed by 4-color cyt...
Jean-Jacques Lataillade et al. Blood 2000;95:756-768
©2000 by American Society of Hematology
SDF-1 increases BFU-E and CFU-G colony formation by PB Inc− CD34+ progenitor cells.PB
CD34+ cells were purified without incubation on a plastic support (Inc−) and plated in duplicate
at a density of 500 cells/mL on semisolid Stem α ID medium containing IL-3...
Jean-Jacques Lataillade et al. Blood 2000;95:756-768
©2000 by American Society of Hematology
SDF-1 increases CFU-G, CFU-M, and immature BFU-E colony formation by PB Inc+ CD34+
progenitor cells.PB CD34+ progenitor cells were purified after incubation on a plastic support
(Inc+) and plated in duplicate, at a density of 500 cells/mL, on semisolid Stem...
Jean-Jacques Lataillade et al. Blood 2000;95:756-768
©2000 by American Society of Hematology
SDF-1 increases the number of megakaryocytic progenitor-derived colonies from PB Inc− and
Inc+ CD34+ cells.PB CD34+ cells were purified (A) without incubation on a plastic support (Inc−)
or (B) after overnight incubation (Inc+) and plated in duplicate at a ...
Jean-Jacques Lataillade et al. Blood 2000;95:756-768
©2000 by American Society of Hematology
Anti–SDF-1 treatment inhibits the stimulatory effect of SDF-1 on colony formation by PB Inc+
CD34+progenitor cells.PB Inc+ CD34+ cells, purified after incubation on a plastic support, were
plated in duplicate at a density of 500 cells/mL on semisolid Stem α...
Jean-Jacques Lataillade et al. Blood 2000;95:756-768
©2000 by American Society of Hematology
SDF-1 increases CFU-G, CFU-M, CFU-GM, and CFU-Mix colony formation by PB Inc+ CD34+
progenitor cells in synergy with SCF.PB CD34+ progenitor cells purified after overnight
incubation on a plastic support (Inc+) were incubated for 48 hours in serum- and cyto...
Jean-Jacques Lataillade et al. Blood 2000;95:756-768
©2000 by American Society of Hematology
SDF-1 increases SCF-induced tyrosine phosphorylation in PB Inc+ CD34+ cells.PB and BM
CD34+ cells purified after incubation on a plastic support (Inc+) were pretreated without (control)
or with SDF-1 (0.05 ng/mL) for 20 hours in serum- and cytokine-free med...
Jean-Jacques Lataillade et al. Blood 2000;95:756-768
©2000 by American Society of Hematology
SDF-1 stimulates colony formation by PB Inc+ CD34+ progenitor cells released from quiescence
by anti–TGF-β antibody treatment.PB Inc+ CD34+ cells, purified after overnight incubation on a
plastic support were incubated for 48 hours in serum-free liquid cult...
Jean-Jacques Lataillade et al. Blood 2000;95:756-768
©2000 by American Society of Hematology