Download syllabus B Sc

Syllabus for B.Sc. III Semester
Class
: B.Sc. II
Subject
: Biotechnology
Semester
: III
Course
: General Biochemistry & Biophysical Techniques
UNIT-I

Introduction and classification of carbohydrate, structure and properties of mono,
oligo and polysaccharides, structure of peptidoglycan,

Introduction and classification of lipid, fatty acid and membrane lipid
UNIT-II

Introduction, classification and importance of protein and amino acid, structure of
protein, hydrophobic and hydrophilic interaction in protein, pH and its measurement.

Nucleic Acid: Introduction, types, chemical and physical properties, structure of BDNA & Z-DNA, structure and function of RNA,

Isotopes, radioactivity, biological use of radioactivity.
UNIT-III

General introduction of enzyme and their classification , mechanism of action,
enzyme kinetics, factors affecting the rate of reaction, enzyme immobilization and
application of enzyme

General idea of vitamins structure, types & function, role of metal ion in Biological
system (K, Na, Mg, Ca & Fe)
UNIT-IV

Energetics of living body, electrical properties of Biological compartments.

Generation and reception of sonic vibrations, hearing aids

Correction of vision faults

Physical methods of imaging intact biological structure ultrasounds, optical filters, Xray, CAT scan, NMR imaging, EEG, ECG
UNIT-V

Lambert- Beer’s Law

General spectroscopy, UV-visible, IR, Raman spectroscopy, X-ray crystallography,
NMR.

Electrophoresis: principle and types (SDS-PAGE, Agarose Gel electrophoresis,
Isoelectrofocusing)

Chromatography: principle and types (Paper, Ion-Exchange chromatography, Thinlayer chromatography)