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Dried Blood Filter Paper Testing: Spot Check Dried Blood Filter Paper Testing: Spot Check Valid Specimen Valid Specimen 100 μl of blood has been collected in each circle, completely saturating or filling the circle and is soaked through to the other side of the card. 100 μl of blood has been collected in each circle, completely saturating or filling the circle and is soaked through to the other side of the card. Collection card has been labeled with patient’s name and date of birth. Collection card has been labeled with patient’s name and date of birth. Invalid specimens Invalid specimens Quantity insufficient Quantity insufficient Circles not completely filled or blood did not soak through; filter paper contaminated with powder or lotion. Circles not completely filled or blood did not soak through; filter paper contaminated with powder or lotion. Not dry before packaging Not dry before packaging Specimen not dried for a minimum of two hours prior to packaging. Specimen not dried for a minimum of two hours prior to packaging. Supersaturated Supersaturated Excess blood applied (usually by capillary or needle) or blood applied to both sides of card. Excess blood applied (usually by capillary or needle) or blood applied to both sides of card. Serum rings Serum rings Alcohol not wiped off puncture site before skin puncture; filter paper contaminated with alcohol, water or lotion; puncture site excessively squeezed; improperly dried; blood applied with capillary tube containing an anticoagulant. Alcohol not wiped off puncture site before skin puncture; filter paper contaminated with alcohol, water or lotion; puncture site excessively squeezed; improperly dried; blood applied with capillary tube containing an anticoagulant. Clotted or layered Clotted or layered Excess blood applied (usually by capillary or needle) several times or blood applied to both sides of card. Excess blood applied (usually by capillary or needle) several times or blood applied to both sides of card. Scratched or abraded Scratched or abraded Blood applied with capillary tube. Blood applied with capillary tube. Diluted, discolored or contaminated Diluted, discolored or contaminated Puncture site squeezed or “milked” to expel blood; exposure to direct heat; filter paper contamination with powder, water, alcohol or lotion. Puncture site squeezed or “milked” to expel blood; exposure to direct heat; filter paper contamination with powder, water, alcohol or lotion.