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Improved separation speed and efficiency for proteins, nucleic acids and viruses in asymmetrical
flow field flow fractionation
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Journal Article
Litzen, A.;Wahlund, K. G.
Improved separation speed and efficiency for proteins, nucleic acids and viruses in
asymmetrical flow field flow fractionation
J. Chromatogr.
J. Chromatogr.
413-21
476
asym field flow fractionation chromatog biomol,protein field flow fractionation
chromatog,virus field flow fractionation chromatog,nucleic acid field flow fractionation
chromatog
1989
The performance of the asym. flow field-flow fractionation channel has been improved by the use of a much thinner
(0.12-mm) channel than before and by flow programming (stepwise gradient elution). The thinner channel
contributes to a decreased zone broadening which enables complete resoln. in a shorter time. Three protein peaks,
representing mol. wts. 12,000-136,000, were completely resolved within 3 min. Flow programming speeds up the
elution of the high-mol.-wt. materials which occur late in a fractogram. This enabled sepn. of a small plasmid
fragment (700 base pairs) from the large amts. of a big fragment (4600 base pairs) in 30 min and improved
detection of a presumed trimer of albumin. Two viruses (1.8 .times. 106 and 50 .times. 106 daltons) were eluted as
narrow peaks within 5 min.
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