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Download Poster Material-Lucas Rizkalla
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Table 1. Endolysin Functional Domains Phage Catalytic Domain Cell Wall Targeting Domain N-acetylmuramoyl-L-alanine amidase D-alanyl-D-alanine carboxypeptidase N-acetylmuramoyl-L-alanine amidase D-alanyl-D-alanine carboxypeptidase N-acetylmuramoyl-L-alanine amidase D-alanyl-D-alanine carboxypeptidase SH3 SH3 SH3 N-acetylmuramoyl-L-alanine amidase D-alanyl-D-alanine carboxypeptidase N-acetylmuramoyl- L-alanine amidase SH3 Waukesha SH3 Gamma N-acetylmuramoyl-L-alanine amidase SPO1 N-acetylmuramoyl-L-alanine amidase D-alanyl-D-alanine carboxypeptidase Spore cortex-lytic enzyme N-acetylmuramoyl-L-alanine amidase Membrane-bound lytic murein transglycosylase D N-acetylmuramoyl-L-alanine amidase Membrane-bound lytic murein transglycosylase D N-acetylmuramoyl-L-alanine amidase Membrane-bound lytic murein transglycosylase D N-acetylmuramoyl-L-alanine amidase Glycosyl Hydrolase 1,4-beta-N-acetylmuramidase (Lyzozyme M1) N-acetylmuramoyl-L-alanine amidase Glycosyl Hydrolase 1,4-beta-N-acetylmuramidase (Lyzozyme M1) N-acetylmuramoyl-L-alanine amidase Glycosyl Hydrolase 1,4-beta-N-acetylmuramidase (Lyzozyme M1) N-acetylmuramoyl-L-alanine amidase N-acetyl-anhydromuranmyl-L-alanine N-acetylmurmoyl-lalanine amidase PGRP Putative peptiodoglycanbinding LysM SafA LysM SafA LysM SafA N-acetylmuramoylL-alanine amidase SH3b B4 Troll Jugalone Phrodo Taylor Curly Gemini Vinny Evoli HoodyT Nigalana N-acetylmuramoylL-alanine amidase SH3b N-acetylmuramoylL-alanine amidase SH3b SH3 PGRP amidase NotTheCreek SageFayge DIGNKC Zuko N-acetylmuramoyl-L-alanine amidase SH3 N-acetyl -anhydromuranmyl-L-alanine PGRP amidase N-acetylmuramoyl-L-alanine amidase SH3 N-acetyl-anhydromuranmyl-L-alanine PGRP amidase N-acetylmuramoyl-L-alanine amidase N-acetyl-anhydromuranmyl-L-alanine amidase N-acetylmuramoyl-L-alanine amidase N-acetyl-anhydromuranmyl-L-alanine amidase 1. N-acetyl muramidase 2. Lytic Transglycosylase 3. N-acetylmuramoyl- L-alanine amidase 7. D-alanyl-D-alanine carboxypeptidase Can’t find N-acetyl-anhydromuranmyl-L-alanine amidase SH3 PGRP SH3 PGRP Endolysin Phylogeny 6 1 2 3 4 5 6 B. cereus=blue B. subtilis=yellow B. pumilus=green B. anthracis=orange B. thuringiensis=purple =Endolysin & Holin close Figure1. The phages were characterized with Clustal multiple sequence alignment, calculated using neighbor joining using BLOSUM62, to create an endolysin phylogenetic tree. 1) Phages B4, Troll, Phrodo, and Jugalone have the same predicted functional domains, with N-acetylmuramoyl-L-alanine amidase and D-alanyl-D-alanine carboxypeptidase at the N-terminal while also having SH3 at its C-terminal. 2) Taylor, Curly, and Gemini also share the same functional domains. Their N-terminal consists of N-acetylmuramoyl-L-alanine amidase and membrane-bound lytic murein transglycosylase D, while its C-terminal consists of LysM and SafA. 3) HoodyT, Vinny, and Evoli all share N-acetylmuramoyl-Lalanine amidase, glycosyl hydrolase, and 1,4-beta-N-acetylmuramidase (Lyzozyme M1) at their N-terminal. Their C-terminals all share functional domains of N-acetylmuramoyl-Lalanine amidase and SH3b. 4) SPO1 stands alone, with N-acetylmuramoyl-L-alanine amidase, D-alanyl-D-alanine carboxypeptidase, and spore cortex-lytic enzyme at its Nterminal and putative peptiodoglycan-binding at its C-terminal. 5) Gamma and Waukesha have the same N-terminal functional domain of N-acetylmuramoyl-L-alanine amidase, but Waukesha has different C-terminal functions. 6) DIGNKC, Zuko, SageFayge, Nigalana, and NotTheCreek share the same N-terminal and C-terminal functional domains. N-Terminal 1 N-acetylmuramoyl-l-alanine amidase D-alanyl-d-alanine carboxypeptidase 2 N-acetylmuramoyl-l-alanine amidase Membrane-bound lytic murein transglycosylase D 3 N-acetylmuramoyl-l-alanine amidase Gylcosyl hydrolase 1,4-beta-N-acetylmuramidase 4 N-acetylmuramoyl-l-alanine amidase D-alanyl-d-alanine carboxypeptidase Spore cortex-lytic enzyme 5 6 N-acetylmuramoyl-l-alanine amidase N-acetylmuramoyl-l-alanine amidase N-acetyl-anhydromuranmyl-l-alanine amidase C-Terminal SH3 LysM SafA LysM SafA N-acetylmuramoyl-lalanine amidase SH3b Putative peptidoglycan binding Putative peptidoglycan binding SH3 (Waukesha) N-acetylmuramoyl-l-alanine amidase (Gamma) PGRP (Gamma) SH3 PGRP
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