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465g. MINERAL MEDIUM WITH CYANURIC ACID AS NITROGEN SOURCE Prepare and autoclave medium 465 without ammonium sulphate and calcium nitrate. Add 5.5 g/l glycerol from a sterile solution. Add 645 mg/l cyanuric acid from a neutralized and filter sterilized stock solution and 10 ml vitamin solution (see medium 461) to the medium. © 2007 DSMZ GmbH - All rights reserved