Download P142_Testarea toxicitatii unor extracte vegetale obtinute din

Toxicity assessment of some extracts from Leucojum vernum
and Narcissus x incomparabilis (Amaryllidaceae)
Oana Cristina Șeremet1, Dinu Mihaela1, Robert Viorel Ancuceanu1, Mădălina Alexandra Rusînac1,
Octavian Tudorel Olaru1
1 – “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania
Objectives
Leucojum vernum (spring snowflake) and
Narcissus x incomparabilis (daffodil) are two
ornamental species belonging to Amaryllidaceae
family, that contain toxic alkaloids (galantamine
and lycorine) for both, animal and plant cells. The
objective of this research is to determine the
toxicity of the two species using two plant cell
models of cytotoxicity assesment (Triticum and
Lactuca bioassays).
A
B
Fig 1. A – Leucojum vernum; B – Narcissus x incomparabilis
Results
Daffodil extract IC50 values were 0.0200 (Triticum
bioassay) and 0.1875 µg/mL (Lactuca bioassay), whereas the
IC50 values for the spring snowflake extract were 0.0469 µg/mL
(Triticum bioassay) and 0.2793 µg/mL (Lactuca bioassay).
Material and methods
The plant extracts were obtained from the
bulbs of the two species, by maceration for 10 days
in ethanol, in the ratio of 1:20 (w/v), followed by
concentration and lyophilization. The toxicity
evaluation was based on the determination of the
inhibitory concentrations which inhibits 50% of the
root elongation (IC50) of Lactuca sativa and
Triticum aestivum, after a 24h period of exposure.
The tested concentrations of extracts were
between 10 and 2000 µg/mL.
Conclusions
Both extracts show
high toxicity, daffodil
extract being more
potent.
a
b
a
b
c
d
Fig 2. Inhibitory effect – logarithm of concentration regression curves: a –
N.xincomparabilis, b – L. vernum (Lactuca bioassay); c – N.xincomparabilis, d – L. vernum
(Triticum bioassay);
References
1. Seremet OC, Olaru OT,
Balalau D, Negres S. The effect
of certain plant extracts
containing
pyrrolizidine
alkaloids on Lactuca sativa
radicle growth. Rom. J. Biopys.
2014; 24 (1): 1-9;
2. Nițulescu GM, Drăghici C,
Olaru OT. New Potential
Antitumor Pyrazole Derivatives:
Synthesis
and
Cytotoxic
Evaluation. : Int. J. Mol. Sci.
2013, 14, 21805-21818 ;
3. Olaru OT, Anghel A, Istudor
V, Ancuceanu R, Dinu M.
Contributions
to
the
Pharmacognostical
and
Phytobiological
study
of
Fallopia aubertii (L.Henry)
Holub.
(Polygonaceae).
Farmacia. 2013;61(5): 991-999
c