Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Toxicity assessment of some extracts from Leucojum vernum and Narcissus x incomparabilis (Amaryllidaceae) Oana Cristina Șeremet1, Dinu Mihaela1, Robert Viorel Ancuceanu1, Mădălina Alexandra Rusînac1, Octavian Tudorel Olaru1 1 – “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania Objectives Leucojum vernum (spring snowflake) and Narcissus x incomparabilis (daffodil) are two ornamental species belonging to Amaryllidaceae family, that contain toxic alkaloids (galantamine and lycorine) for both, animal and plant cells. The objective of this research is to determine the toxicity of the two species using two plant cell models of cytotoxicity assesment (Triticum and Lactuca bioassays). A B Fig 1. A – Leucojum vernum; B – Narcissus x incomparabilis Results Daffodil extract IC50 values were 0.0200 (Triticum bioassay) and 0.1875 µg/mL (Lactuca bioassay), whereas the IC50 values for the spring snowflake extract were 0.0469 µg/mL (Triticum bioassay) and 0.2793 µg/mL (Lactuca bioassay). Material and methods The plant extracts were obtained from the bulbs of the two species, by maceration for 10 days in ethanol, in the ratio of 1:20 (w/v), followed by concentration and lyophilization. The toxicity evaluation was based on the determination of the inhibitory concentrations which inhibits 50% of the root elongation (IC50) of Lactuca sativa and Triticum aestivum, after a 24h period of exposure. The tested concentrations of extracts were between 10 and 2000 µg/mL. Conclusions Both extracts show high toxicity, daffodil extract being more potent. a b a b c d Fig 2. Inhibitory effect – logarithm of concentration regression curves: a – N.xincomparabilis, b – L. vernum (Lactuca bioassay); c – N.xincomparabilis, d – L. vernum (Triticum bioassay); References 1. Seremet OC, Olaru OT, Balalau D, Negres S. The effect of certain plant extracts containing pyrrolizidine alkaloids on Lactuca sativa radicle growth. Rom. J. Biopys. 2014; 24 (1): 1-9; 2. Nițulescu GM, Drăghici C, Olaru OT. New Potential Antitumor Pyrazole Derivatives: Synthesis and Cytotoxic Evaluation. : Int. J. Mol. Sci. 2013, 14, 21805-21818 ; 3. Olaru OT, Anghel A, Istudor V, Ancuceanu R, Dinu M. Contributions to the Pharmacognostical and Phytobiological study of Fallopia aubertii (L.Henry) Holub. (Polygonaceae). Farmacia. 2013;61(5): 991-999 c