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From: Sclera as a Surrogate Marker for Determining AGE-Modifications in Bruch's Membrane Using a Raman
Spectroscopy–Based Index of Aging
Invest. Ophthalmol. Vis. Sci.. 2011;52(3):1593-1598. doi:10.1167/iovs.10-6554
Figure Legend:
Raman spectra of fatty acid lipids from (a) sclera, (b) Bruch's membrane, and (c) lung surfactant compared with Raman
signals from pure fatty acid methyl esters (d), oleic (18:1c; unsaturated, liquid), (e) palmitic (16:0; saturated, solid), and
(f) scleral carotenoid compared with (g) beta carotene. All spectra were recorded at 633 nm at 21°C. Spectral intensities
were normalized by the mean intensity of the spectra. Spectra were offset for clarity. Bandwidth of the features was
dependent on the physical state—sharp in solids, broad in liquids. Bruch's membrane fat has a low solid fat content with
significant unsaturation, whereas scleral fat has a high solid fat content and low saturation.
Date of download: 7/31/2017
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