Download Determination of growth inhibitory activities of several bioactive

SAMPLE ABSTRACT
Determination of growth inhibitory activities of several bioactive
compounds of Andrographis paniculata against a panel of human tumor
cell lines: 14-deoxy-11, 12-didehydroandrographolide induces a nonapoptotic programmed cell death in T-47D, a breast carcinoma cell line
Tan Mei Lan1, Tengku Sifzizul Tengku Muhammad1, Masanori Kuroyanagi2, Shaida
Fariza Sulaiman1, Nazalan Najimudin1
1
School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia. 2School of
Bioresources, Hiroshima Prefectural University, Shobara-shi, Hiroshima 727 Japan.
e-mail: [email protected]
The growth inhibitory activities of seven bioactive compounds of the medicinal plant,
“Hempedu Bumi” or Andrographis paniculata against a panel of five human tumor cell lines
were evaluated. The seven compounds used were andrographolide, 14-deoxyandrographolide,
andrographiside, deoxyandrographiside, 14-deoxy-12-methoxyandrographolide and 14deoxy-11, 12-didehydroandrographolide against Caov-3 (human ovarian carcinoma) cell
lines, T-47D (human breast carcinoma) cell lines, Hs-578T (human breast carcinoma) cell
lines, Hep G2 (human hepatocarcinoma) cell lines and NCI-H23 (human lung
adenocarcinoma) cell lines. Cell survival was carried out using the MTS assay after 72 hours
of incubation. Andrographolide, andrographiside, deoxyandrographiside and 14-deoxy-12methoxyandrographolide appeared to be non-cytotoxic against all the cell lines, as judged by
the criterion set by the National Cancer Institute, USA (EC50 was more than 4 µg/ml).
However, only 14-deoxyandrographolide and 14-deoxy-11, 12-didehydroandrographolide
exhibited prominent cytotoxic activities but was only limited to T-47D cell line (EC50 at
2.800 µg/ml and 1.520 µg/ml, respectively). As principle compounds of A. paniculata, 14Deoxy-11, 12-didehydroandrographolide appeared to be the most potent as compared to the
rest of the compounds. The effects of 14-deoxy-11, 12-didehydroandrographolide on T-47D
cells was further confirmed to be non-apoptotic, non-necrotic but programmed in nature as
demonstrated using a modified TUNEL assay, trypan blue exclusion assay and annexin Vpropidium iodide staining.