Download osswald - blumenroether

Experiment 1 (June, August, September 2003)
sun
shade
Samples: per branch (sun/shade) 5 samples (consisting of several leaves)
will be measured seperately;
samples will be collected between 10-1100 once in
June, August, September
Measurements: (June, August, September 2003)
leaf weight (fresh/dry) & area
total protein content
sugars
enzyme activity for Rubisco and PEPC
chlorophyll content
Sample number:
5 (samples per tree branch) x 2 (sun/shade) x 10 (5 +O3/5 –O3) x 3 = 300
Expected results:
Experiment 2 (August 2003)
sun
shade
Samples:5 to 10 leaves per branch (sun/shade)
pooled as one sample taken at different
times per day
Expected results:
8
enzyme activity
Measurements: (August 2003)
leaf weight (fresh/dry) & area
total protein content
sugars
enzyme activity for Rubisco
and PEPC
chlorophyll content
7
6
?
5
4
3
2
1
Sample number:
2 (sun/shade) x 6 (time points) x 10 (5 +O3/5 –O3) =
= 120
0
6.00
9.00
12.00
15.00
hour
18.00
21.00
Experiment 3 (August 2004)
Samples:
taken from a highly O3 sensitive tree, from a
less sensitive tree and from a control tree
in total three trees
sun and shade branches
taken at 7/12/15/19/22/1 o‘ clock during day
Measurements:
gene expression for Rubisco (rbcS/rbcL),
Osmotin and PR1 protein
sugar contents
Rubisco activities
Sample number: 3 (trees) x 2 (sun/shade) x 6 (time points) = 36