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DR SONALI SHARMA LECTURE SUMMARY REPLICATION – Dated :13-1-16 Gene,genome Meselson Stahl experiment Process of replication in prokaryotes Replication fork,DNA polymerases Topoisomerases Eukaryotic DNA replication Inhibitors of replication Cell cycle & replication REPLICATION – Dated : 20-1-16 ; Telomeres & telomerase Higher organisation of DNA Recombination DNA damage Repair mechanisms Diseases associated with defective repair DR Manju jha Lecture summary of the class to be held on 06-06-2016 ELECTROPHORESISDEFINITION FACTORS AFFECTING ELECTROPHORESIS TYPES OF ELECTROPHORESIS PAPER ELECTROPHORESIS GEL ELECTROPHORESIS PAGE SDS-PAGE ELECTROPHORESIS AS AN ANALYTICAL TOOL Dr sunil Gupta Lec.Date-7-1-15&9-1-15 Nutrition- SUMMARY • Introduction, macro and micro nutrients • Nutritional importance of carbohydrates,fats,proteins&amino acids • Respiratory quotient of food stuff,basal metabolic rate,specific dyanemic action • Balanced diet, dietary fiber • Nutritional disorder include kwashiorkor and marasmus • Obesity DIETARY FIBER- SUMMARY Lec.Date-18-01-16 • Defination,sources,beneficial and adverse effects of dietary fiber DR Priyanka jain lecture summary TRANSCRIPTION 21-1-16 STRUCTURE OF RNA (r RNA, t RNA & m RNA) TRANSCRIPTION OF PROKARYOTIC GENES 1. Properties & structure of prokaryotic RNA polymerase 2. Steps in RNA synthesis (Initiation - –35 sequence & pribnow box, elongation & termination- ᵽ Independent termination, ᵽ dependent termination) 3. Action of antibiotics TRANSCRIPTION 23-1-16 TRANSCRIPTION OF EUKARYOTIC GENES A. Chromatin structure and gene expression B. Nuclear RNA polymerases of eukaryotic cells 1. RNA polymerase I 2. RNA polymerase II (Promoters and transcription factors for RNA polymerase II, Role of enhancers in eukaryotic gene regulation, Inhibitors of RNA polymerase II) 3. RNA polymerase III MITOCHONDRIAL RNA POLYMERASE POSTTRANSCRIPTIONAL MODIFICATION OF RNA A. Ribosomal RNA B. Transfer RNA C. Eukaryotic mRNA: 1. 5' “Capping” 2. Addition of a poly-A tail 3. Removal of introns (Role of snRNAs, Mechanism of splicing, Effect of splice site mutations) 4. Alternative splicing of mRNA molecules: