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PA1181 US office: Acris Antibodies, Inc. San Diego, CA UNITED STATES Phone: +1-858-888-7900 Fax: +1-858-888-7904 [email protected] Acris Antibodies GmbH Schillerstr. 5 32052 Herford GERMANY Phone: +49-5221-34606-0 Fax: +49-5221-34606-11 [email protected] Human Chorionic Gonadotropin (hCG) Alternate names: Choriogonadotropin subunit beta, CGB, CGB3, beta hCG, hCG-beta Catalog No.: PA1181 Quantity: 10 µg Background: Human chorionic gonadotropin (hCG) is a peptide hormone produced in pregnancy, that is made by the embryo soon after conception and later by the syncytiotrophoblast (part of the placenta). Its role is to prevent the disintegration of the corpus luteum of the ovary and thereby maintain progesterone production that is critical for a pregnancy in humans. hCG may have additional functions, for instance it is thought that it affects the immune tolerance of the pregnancy. Early pregnancy testing generally is based on the detection or measurement of hCG. Uniprot ID: P01233 Species: Human Source: CHO cells Chinese Hamster Ovary Cells (CHO) Format: State: Sterile filtered, white lyophilized (freeze-dried) powder Purity: >99% Purified by proprietary chromatographic techniques. Purity > 99.0 %, determined by: (a) Analysis by RP-HPLC, (b) Analysis by SDS-PAGE Buffer System: Lyophilized from a concentrated (1 mg/ml) solution containing 110 mg mannitol, 1.7 mg phosphoric acid, 0.4 mg L-Methionine and 0.2 mg poloxamer pH 7. Reconstitution: Reconstitute in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Description: Human Chorionic Gonadotropin Recombinant produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single glycoprotein chain consisting of two non-covalently linked subunits - designated alpha and beta consisting of 92 and 145 amino acid residues (237 a.a. total, Mw: 36.7kDa), respectively, with carbohydrate moieties linked to ASN-52 and ASN-78 (on alpha subunit) and ASN-13, ASN-30, SER-121, SER-127, SER-132 and SER-138 (on beta subunit). The primary structure of the alpha chain of r-hCG is identical to that of the alpha chain of hCG, FSH and LH. The glycoform pattern of the alpha subunit of r-hCG is closely comparable to urinary derived hCG (u-hCG), the differences mainly being due to the branching and sialylation extent of the oligosaccharides. The beta chain has both O and N-glycosylation sites and its structure and glycosylation pattern are also very similar to that of urinary hCG. Biological Activity: The biological activity of choriogonadotropin alpha is determined by using the seminal vesicle weight gain test in male rats described in the "Chorionic Gonadotrophins" monograph of the European Pharmacopoeia. The in vivo biological activity of choriogonadotropin alpha has been calibrated against the third international reference preparation IS75/587 for chorionic gonadotropin. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. AV/20081126 Antibody Hotline - Technical Questions - Antibody Location Service Free Call: 0800-2274746 (Germany only) - www.acris-antibodies.com 1/2 PA1181: Human Chorionic Gonadotropin (hCG) Specific Activity: - 26,000 IU/mg Molecular weight: 37 kD Storage: Lyophilized hCG, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CG-beta should be stored at 2-8°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. Shelf life: one year from despatch. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. AV/20081126 Antibody Hotline - Technical Questions - Antibody Location Service Free Call: 0800-2274746 (Germany only) - www.acris-antibodies.com 2/2