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Freeze-Fracture TEM
Liposome, Biological Samples, Foods, Health-Care Products…
Freeze-fracture techniques are based on the mechanical fracture of frozen material and
the high-resolution metal foil replication of the exposed surfaces. Replication by vapor
deposition of platinum and carbon reveals nano-structures of membrane, interface and
dispersion state of materials by transmission electron microscopy (TEM) at high resolution.
Procedure of Freeze-Fracture Replica Transmission Electron Microscopy (FF-TEM)
Pt
Freeze
Freeze Fracture
(FF)
C
Pt coating
(Shadowing)
Carbon coating
Replica film
Application Fields
Biological samples
Foods
mayonnaise
cheese
ice cream
butter
chocolate
food oil
Freeze-Fracture Apparatus (JEOL JFDII)
cell
DNA
virus
bacteria
myosin
protein
To TEM
Materials
emulsion
gel
ink
pigment
oil
liposome
Applications of Freeze-Fracture Technique
Freeze-Fracture Replica TEM
500nm
100nm
200nm
Milk
Emulsion
Freeze-Etching Replica TEM
Liposome
Freeze-Fracture Replica
Ice
sublimation
2µm
2µm
Hair Conditioner
100nm
Liposome (Negatively Stained)
Toray Research Center, Inc.
S00193有機分析第２研究室20100809 STC：開(20141121)
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