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Ch. 39 Warm-Up 1. Elaborate on the methods plants use to defend themselves from pathogens and herbivores. 2. How do plants cope with: a. Flooding b. Drought c. Heat stress d. Salt stress 3. If a long day plant needs at least 9 hours of dark, which scenario(s) will prevent flowering? a. 16 hours light, 8 dark b. 14 hours light, 10 dark c. 14 hours light, 10 dark w/ flash of light Ch. 39 Warm-Up Match the following terms with the descriptions below: A. Auxin B. Cytokinins C. Gibberellins D. Abscisic Acid E. Ethylene 1. 2. 3. 4. 5. Inhibits growth, closes stomata during H2O stress Fruit ripening, promotes or inhibits growth Affects root growth, stimulates cell division & growth, stimulates germination Stimulates stem elongation, root growth, fruit development, photo- & gravitropism Promotes seed & bud germination, stem elongation, flowering & development of fruit What you must know: The three steps to a signal transduction pathway. The role of auxins in plants. How phototropism and photoperiodism use changes in the environment to modify plant growth and behavior. How plants respond to attacks by herbivores and pathogens. Chapter 39 Plant Responses to Internal and External Signals Experiments with Light and the coleoptile Conclusion: Tip of coleoptile responsible for sensing light, but growth occurred below tip some signal was sent from tip to elongating region of coleoptile Excised tip placed on agar block Cells on darker side elongate faster than cells on brighter side AUXIN = chemical messenger that stimulates cell elongation Growth-promoting chemical diffuses into agar block Control (agar block lacking chemical) has no effect Control Agar block with chemical stimulates growth Offset blocks cause curvature Hormones: chemical messengers that coordinate different parts of a multicellular organism Important plant hormones: 1. Auxin – stimulate cell elongation phototropism & gravitropism (high concentrations = herbicide) 2. Cytokinins – cell division (cytokinesis) & differentiation 3. Gibberellins – stem elongation, leaf growth, germination, flowering, fruit development 4. Abscisic Acid – slows growth; closes stomata during H2O stress; promote dormancy 5. Ethylene – promote fruit ripening (positive feedback!); involved in apoptosis (shed leaves, death of annuals) The effects of gibberellin on stem elongation and fruit growth Ethylene gas: fruit ripening Canister of ethylene gas to ripen bananas in shipping container Untreated tomatoes vs. Ethylene treatment Plant Movement 1. Tropisms: growth responses SLOW Phototropism – light (auxin) Gravitropism – gravity (auxin) Thigmotropism – touch 2. Turgor movement: allow plant to make relatively rapid & reversible responses Venus fly trap, mimosa leaves, “sleep” movement Positive gravitropism in roots: the statolith hypothesis. Thigmotropism: rapid turgor movements by Mimosa plant action potentials Plant Responses to Light Plants can detect direction, intensity, & wavelenth of light Phytochromes: light receptors, absorbs mostly red light ◦ Two forms: Pr (red light) and Pfr (far-red light) ◦ Pr Pfr: switches depending on light in greatest supply ◦ Pfr aids in detection of sunlight ◦ Regulate seed germination, shade avoidance Biological Clocks Circadian rhythm: biological clocks Persist w/o environmental cues Frequency = 24 hours Phytochrome system + Biological clock = plant can determine time of year based on amount of light/darkness Sleep movements of a bean plant. Caused by reversible changes in turgor pressure of cells on opposing sides of the pulvini, motor organs of the leaf. Photoperiodism: physiological response to the relative length of night & day (i.e. flowering) Short-day plants: flower when nights are long (mums, poinsettia) Long-day plant: flower when nights are short (spinach, iris, veggies) Day-neutral plant: unaffected by photoperiod (tomatoes, rice, dandelions) Night length is a critical factor! How does interrupting the dark period with a brief exposure to light affect flowering? Plant Response to Stress Causes of stress: 1. 2. 3. 4. 5. 6. 7. Drought (H2O deficit) Flooding (O2 deprivation) Salt excess Heat Cold Herbivores Pathogens 1. H2O deficit: close stoma release abscisic acid to keep stoma closed Inhibit growth roll leaves reduce SA & transpiration deeper roots 2. Flooding (O2 deprivation): release ethylene root cell death air tubes formed to provide O2 to submerged roots 3. Salt: cell membrane – impede salt uptake produce solutes to ↓ψ - retain H2O 4. Heat: evap. cooling via transpiration heat shock proteins – prevent denaturation 5. Cold: alter lipid composition of membrane (↑unsat. fatty acids, ↑fluidity) increase cytoplasmic solutes antifreeze proteins 6. Herbivores: physical (thorns) chemicals (garlic, mint) recruit predatory animals (parasitoid wasps) 7. Pathogens: 1st line of defense = epidermis 2nd line = pathogen recognition, host-specific