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Download Figure 09: Phage T4 life cycle
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Figure 09: Phage T4 life cycle Adapted from K. Thiel, Nat. Biotechnol. 22 (2004): 31-36. Figure 03B: Phage T4 plaques on a bacterial lawn Part B © Ken Wagner/Visuals Unlimited λ can enter the ly+c or lysogenic cycle Figure 8.28 Adapted from A. Campbell, Nat. Rev. Genet. 4 (2003): 471-‐477. Figure 9.12 RNA ss MS2 ds φ6 ssDNA φψ174 fd, M13 dsDNA T3, T7 Mu Lambda T2, T4 Figure 19: Map of Phage φX174 Adapted from an illustration by New England BioLabs, Inc. Figure 31: Electron micrograph of bacteriophage P1. Photo courtesy of Michel Wurtz and the Biocenter at the University of Basel Figure 10.14 Lytic cycle Phage Normal lytic events Phage DNA Host DNA Donor cell Normal phage Transduction Recipient cell Recipient infected by transducing particle Homologous recombination Transduced recipient cell Transducing particle (contains donor cell DNA) Generalized transducing par+cles carry random bacterial genes Figure 8.32 Figure 9.16 Temperate virus Host DNA Viral DNA Attachment Cell (host) Injection Lytic pathway Lysogenic pathway Viral DNA replicates Induction Coat proteins synthesized; virus particles assembled Viral DNA is integrated into host DNA Lysogenized cell Prophage Lysis Cell division Figure 9.18 cos att Lambda genome Cyclizes at cohesive ends cos Host genes near attachment site att bio gal moa Host DNA Site-specific nuclease creates staggered ends of phage and host DNA bio gal moa Integration of lambda DNA and closing of gaps by DNA ligase gal cos bio moa λ phage is a versa+le cloning vector Figure 8.30 Nonessen+al gene region can be replaced by 12-‐22 kb of foreign DNA.
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