Download fully automated madin-darby canine kidney (mdck)

ROBOTICS APPLICATION SOLUTION
Drug Discovery Solution:
FULLY AUTOMATED MADIN-DARBY CANINE KIDNEY (MDCK)
PERMEABILITY ASSAY
Permeability assays elucidate the structure-activity relationships in the hit-to-lead stage, rank order compounds
for in vivo pharmacokinetics studies and predict the potential for oral absorption. Reliability, accuracy and
precision are critical for these assays, particularly when they are used in medium- and high-throughput
screening.
Madin Darby Canine Kidney (MDCK) cells are a common model for studying drug transport mechanisms in
distal renal epithelia. MDCK cells grown to confluence on permeable membranes are widely used to examine
passive permeability and active transport. These assays have typically been run manually or have been partially
automated, requiring an operator’s presence at all times. Hamilton Robotics successfully automated the 24-well
MDCK permeability assay using the MICROLAB® STAR Automated Liquid Handling Workstation, integrated with
select third-party peripheral devices.
System Features:
•
•
•
BioTek plate reader
Thermo Cytomat incubator
Hamilton CO-RE Grippers
Deck Layout:
System and Materials:
System Requirements
Hamilton Part Number
MICROLAB STAR with Autoload, 12 independent channels, iSWAP microplate
gripper, CO-RE 96 Probe Head
CO-RE Gripper
VENUS one software
DWP Carrier (five SBS positions)
MTP Carrier (five SBS positions)
Tip Carrier (five 96 Tips)
Multiflex Carrier Base (Landscape Orientation)
Multiflex Tip Module
Multiflex Tilt module
Multiflex DWP Module x 3
Labware Required
173000-027
184089
911004-V1, 911004
182090
182085
188160
188039
188160
188061APE
188042
Hamilton Part Number
50 µL CO-RE disposable tips without filters
300 µL CO-RE disposable tips without filters
1000 µL CO-RE disposable tips without filters
235966 (black conductive)
235902 (black conductive)
235904 (black conductive)
User-supplied Materials
Part Number
Millipore Millicell 24-well Cell Culture Plate
BD Falcon Flat Bottom UV Plates w/lid
E&K Scientific 12-Column Reservoirs, Deep Well, Pyramid Bottom
Axygen 12-Column Reservoirs, Low Profile, Partitioned
Seahorse Bioscience Custom 36-well Deep Well Plate
Thermo Scientific Matrix 2D Bar-coded ScrewTop Storage Tubes & Caps: 500
μl 2D Bar-coded, V Bottom ScrewTop Tubes w/ Caps, Sterile
PSRP010R5
353293
EK-2033
Axygen RES-MW-12-LP
S30052
3744
Data:
The figure below shows the correlation of the compounds
tested in a 24-well format using MDCK cells and the
published Papp data. The grouping classifies the
compounds into low, medium and high Papp categories,
and the data suggests that there is a good correlation
between published data and results obtained from the
MDCK assay automated on the Hamilton STAR platform.
Data provided by Plise et al. (Genentech, Inc. South San Francisco, USA).
For more information, please visit www.hamiltonrobotics.com or contact your regional sales manager.
MICROLAB is a registered trademark of Hamilton Robotics
Lit. No. AS005 Hamitlon Robotics Reno, NV 06/11