Download Viruses Chapter 13 (pp. 369 - 396)

Microbiology Student Outline - Viruses
Viruses
Chapter 13 (pp. 369 - 396)
1.
Viruses
•
Obligate Intracellular Parasites (p. 370)
•
Host Range
*
A.
B.
Bacteriophages (Phages)
Results
i.
Cell Death
ii.
Cell Proliferation
iii.
Altered Cell Differentiation
iv.
No Change
Anatomy of a Viron Particle (p. 371ff)
i.
Nucleocapsid
a.
Nucleic Acid
•
dsDNA
•
ssDNA
•
dsRNA
•
ssRNA
•
Segmented Genome
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Microbiology Student Outline - Viruses
b.
c.
Capsid (p, 371)
•
Capsomeres
•
Enzymes
•
Spikes
*
Hemagglutinin
*
Neuraminidase
Envelope
•
Nonenveloped Viruses
•
Enveloped Viruses
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Microbiology Student Outline - Viruses
d.
Viron Morphology (p. 373)
•
Helical
*
•
Rabies
Polyhedral
*
Icosahedral
√
•
Complex
*
e.
Polio Virus
Bacteriophages
Viral Culturing (p. 379)
•
Animal Viruses
*
Living Animals
*
Embryonated Eggs
*
Tissue Culture
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Microbiology Student Outline - Viruses
2.
General Viral Life Cycle (Pull out handout on Viral Lifecycle) (pp. 381 - 392)
A.
Adsorption (Attachment)
B.
Penetration
i.
Engulfment (receptor mediated endocytosis)
ii.
Fusion of Envelope
iii.
Injection
C.
Uncoating and Nucleic Acid Release
D.
Replication (Biosynthesis)
i.
Eclipse Period
E.
Assembly (Maturation)
F.
Release
i.
Lysis
ii.
Budding
iii.
Exocytosis
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Microbiology Student Outline - Viruses
Absorption and
Penetration
Maximum Growth Rate
Viral
Particles
Growth Rate
in Rapid
Log Growth
Decline
Eclipse Period
Time
107
Absorption and
Penetration
Maximum Growth Rate
106
Growth Rate
in Steady
105
4
Viral 10
Particles 103
102
Decline
Log Growth
Eclipse Period
101
100
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28
Time in Days
Adapted from: http://www.nature.com/nri/journal/v5/n10/fig_tab/nri1706_F2.html
3.
Viral Effects
A.
Inclusion Bodies
B.
Block Synthesis in Host
C.
Asymptomatic Latent Viral Infection
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Microbiology Student Outline - Viruses
4.
Bacteriophages (p. 381 - 384)
A.
B.
Morphology
i.
Filamentous
ii.
Complex
Alternation of Lytic and Lysogenic Cycle (see also Bacterial Genetics Handout)
i.
Lytic Cycle
ii.
Lysogenic Cycle
a.
iii.
C.
D.
Temperate Phage (Prophage)
Phage Conversion (or Lysogenic Conversion)
Temperate Phages
i.
Corynebacterium diphtheriae (Diphtheria)
ii.
Streptococcus pyogenes (Scarlet Fever)
iii.
Clostridium botulinum (Botulism)
Temperate Phages and Specialized (Restricted) Transduction
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Microbiology Student Outline - Viruses
5.
RNA Viruses (p. 388 - 390) (See handout on Viruses)
A.
ssRNA Viruses
i.
Sense (+) Strand  Antisense (-) Strand
a.
ii.
Antisense (-) Strand  Sense (+) Strand
a.
B.
Reoviridae (R: respiratory; E: enteric; O: orphan)
Segmented Genome
a.
6.
Rhabdoviridae (Rabies)
dsRNA Viruses
a.
C.
Polio
Influenze
Retro Viruses (p. 390 - 392)
A.
Reverse Transcriptase (RNA  DNA)
B.
Provirus
C.
Budding
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Microbiology Student Outline - Viruses
7.
Cancer and Viruses (pp. 392 - 394)
A.
Oncogenes
B.
Oncogenic Virus
C.
Transformation
D.
DNA Oncogenic Virus Example: Hepatitis B  Liver Cancer
8.
Phage Therapy
xx.
Latent Viral Infections (p. 392)
A.
Herpes Virus
i.
Fever Blisters
B.
Varacella-Zoster
Prions (p. 392 - 393)
A.
Examples
i.
Mad Cow Disease
ii.
Creutzfeldt-Jakob Disease (CJD)
B.
Mechanism
xx.
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