Download Cytoskelton Biochemistry

M1 – Biochemistry
Cytoskelton
Biochemistry
Dr. Diegelmann
1
Cell Surface
Receptors
Collagen
Fibronectin
Integrin Receptors
Cell Membrane
Focal Adhesion
2
Cytoskeleton Biochemistry
3
COMPOSITION & CHARACTERISTICS
The Cytoskeleton is composed of threetypes of protein structures:
1) Actin filamentsÉÉ c omposed of actin
2) MicrotubulesÉ . composed of tubulin
3) Intermediate filamentsÉ .. composed of fibrous proteins such as
vimentin & lamin
Plasma membrane with
supporting protein fibers
3)
Intermediate
filaments
1) Actin
filaments
Nucleus
Nucleu
s
Nuclear lamina
Centrosome
2)
Microtubules
Centrioles
Figure 1 shows the spatial relationships among the 3 m ajor cytoskeletal protein types. The
thin Actin filaments are most highly concentrated in the cortex, just beneath the p lasma
membrane. The Microtubles have one end attached to an o rganizing center called a
Centrosome. The Intermediate filaments can form a Nuclear lamina at the inner surface of
the nuclear membrane and can also be found extending across the cytoplasma from one cellcell junction to another.
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Plasma Membrane
5
Membrane Proteins
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Spectrin Molecule
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Intermediate Filaments
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Nuclear Lamina
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Intermediate Filaments
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Intermediate Filaments
Type of IF
Protein Name
Cellular Location
Nuclear Lamins
Lamins A, B & C
Nuclear lamina
Vimentin-like
Vimentin
Desmin
Glial fibrillary
Peripherin
Many cells
Muscle
Glial cells (astrocytes & Schwann)
Neurons
Keratins
Types I & II
Epithelial-derived cells, hair & nails
Neuronal
NF-L, NF-M & NF-H
Neurons
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Intermediate Filaments
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Ultra Structure
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Keratin Filaments
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Microtubules
15
Polymerization
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Examples of Nucleators
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Cilia
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Cilium or Flagellum
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Centrosome
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Motor Proteins
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Vesicle Transport
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Kineisns & Dyneins
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Microtubules
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Actin
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Microspikes & Filopodia
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Filopodium
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Microvilli
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Myosin-II
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Cell Surface
Receptors
Collagen
Fibronectin
Integrin Receptors
Cell Membrane
Focal Adhesion
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