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CS/B.Sc.(H)/Mol.Bio/SEM-2/GGR-204/2013
2013
GENE EXPRESSION & GENE REGULATION
Time Allotted : 3 Hours
Full Marks : 70
The figures in the margin indicate full marks.
pap
Candidates are required to give their answers in their own words
as far as practicable.
GROUP – A
1.
wb
er.
( Multiple Choice Type Questions )
Choose the correct alternatives for the following :
i)
promoter
b)
transcription unit
c)
terminator region
d)
central region.
The binding of
a)
factor
b)
subunit
c)
subunit
d)
subunit.
RNA
in
a c.
ut.
a)
controlled by
2706
1 = 10
The sequence that will be copied in the form of RNA is
called
ii)
10
polymearse
to
promoter
is
[ Turn over
CS/B.Sc.(H)/Mol.Bio/SEM-2/GGR-204/2013
iii)
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The protein which helps to terminate the transcription
is
iv)
2706
t-RNA
c)
pre m-RNA
d)
small r-RNA.
Enhancers are
a)
proteins located adjacent to promoters
b)
distant sites where regulatory proteins bind
c)
proteins that bind with repressors, deactivating them
d)
expediters of RNA polymerase capture.
All of the following can be found in a huaman
transcription complex except
a)
activator
b)
RNA
c)
enhancer
d)
silencer.
The genetic code contains .................. codons.
a)
4
b)
16
c)
20
d)
64.
in
a c.
ut .
vii)
b)
wb
er.
vi)
rho
pap
v)
a)
In eukaryotes, the transcripton takes place in
a)
nucleus
b)
plasma membrane
c)
cell wall
d)
cytoplasm.
2
CS/B.Sc.(H)/Mol.Bio/SEM-2/GGR-204/2013
viii) In translation, the start codon is
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htt
ix)
UAA
b)
UGA
c)
AUG
d)
UAG.
Nucleosomes inhibit
a)
activators
b)
RNA polymerase
c)
translation
d)
assembly of transcription factors.
A form of binding motif containing a nearly identical
sequence of 60 amino acids in many eukaryotes is the
pap
x)
a)
homeodomain motif
b)
zinc finger motif
c)
leucine zipper motif
d)
universal motif.
wb
er.
a)
GROUP – B
( Short Answer Type Questions )
Answer any three of the following.
3
5 = 15
Describe the Rho independent termination of transcription.
3.
Write a short note on m-RNA processing.
4.
What is the significance of the Genetic code ?
5.
What is codon bias ?
6.
Briefly describe the post-transcriptional modification of
eukaryotic mRNA.
7.
in
a c.
ut .
2.
What do you mean by charging of t-RNA ?
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3
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CS/B.Sc.(H)/Mol.Bio/SEM-2/GGR-204/2013
GROUP – C
/q
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htt
( Long Answer Type Questions )
Answer any three of the following.
8.
How
is
the
process
of
transcription
3
15 = 45
controlled
by
hormones ? How is gene expression regulated by light in
plant cells ? Briefly describe the steps of PCR.
9.
5+5+5
What is meant by a polycistronic transcript, and how is it
related to the lac operon ? What is the normal function of
the operator site and what is the effect of loss of that
?
Starting
with
lactose
pap
function
in
the
extracellular
environment, describe all of the steps that must occur to
induce enhanced expression of the genes in the lac operon.
2+2+2+2+7
10. How does the interaction between the repressor protein
wb
er.
and the operator site differ between the lac operon and the
trp operon ? Describe two distinctly ways in which the
trp operon is controlled by the overall availability of
1
1
tryptophan ?
6+4 +4
2
2
11. Define RNA polymerase. Name the different kinds of RNA
polymerase of eukaryotes and describe
How
is
transcription
eukaryotes ?
role
terminated
in
in
in
a c.
ut .
transcription.
their
3+7+5
12. Define translation. Describe the sequence
during
the
formation of initiation complex. Comment on the role of
ribosome during translation. Give the schematic diagram of
reaction catalyzed by peptidyl Transferase in translation.
2+5+3+5
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4
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CS/B.Sc.(H), Mol-Bio/SEM-2/GGR-204/2012
2012
/qp
GENE EXPRESSION AND GENE REGULATION
Time Allotted : 3 Hours
Full Marks : 70
The figures in the margin indicate full marks.
Candidates are required to give their answers in their own words
ape
as far as practicable.
GROUP – A
( Multiple Choice Type Questions )
Choose the correct alternatives for the following : 10 × 1 = 10
i)
4
c)
20
b)
16
d)
64.
In eukaryotes, the transcription takes place in
a)
nucleus
c)
plasma membrane
b)
d)
cell wall
cytoplasm.
Example of antibiotic inhibitor of transcription is
a)
ampicillin
b)
c)
rifampicin
d)
kanamycin
.in
2706
a)
t.ac
iii)
The genetic code contains ................ codons.
bu
ii)
r.w
1.
tetracycline.
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CS/B.Sc.(H), Mol-Bio/SEM-2/GGR-204/2012
iv)
A dipeptidyl t-RNA is produced by catalyzing the enzyme
htt
v)
a)
peptidyl transferase
b)
DNA ligase
c)
RNA polymerase
d)
amino peptidase.
Translocase is one type of
p:/
initiation factor
b)
release factor
c)
elongation factor
d)
protein.
In translation, the start codon is
a)
c)
vii)
/qp
vi)
a)
UAA
b)
AUG
UGA
d)
UAG.
The release factor (RF1) recognizes
ape
a)
UAA
c)
GCU
b)
AUG
d)
UAC.
viii) In Protein synthesis streptomycin inhibits
initiation and causes misreading of m-RNA
c)
elongation
d)
termination.
The example of protein synthesis inhibitors is
a)
ampicillin
c)
kanamycin
b)
tetracycline
d)
rifampicin.
t.ac
Nucleosomes inhibit
a)
activators
b)
RNA polymerase
c)
translation
d)
assembly of transcription factors.
2
.in
2706
b)
bu
x)
initiation
r.w
ix)
a)
CS/B.Sc.(H), Mol-Bio/SEM-2/GGR-204/2012
GROUP – B
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htt
( Short Answer Type Questions )
3 × 5 = 15
Answer any three of the following.
Write the characteristics and significance of Genetic Code.
3.
Describe briefly the initiation step of protein synthesis.
4.
What is Wobble hypothesis ?
5.
What is the significance of Enhancer sequences in eukaryotic
gene regulation ?
6.
Describe the Rho dependent transcription termination in
detail.
pap
2.
GROUP – C
( Long Answer Type Questions )
3 × 15 = 45
7.
wb
er.
Answer any three of the following.
What do you mean by transcription ? Differentiate between
prokaryotic and eukaryotic transcription. Give the location
and function of different classes of RNA molecule.
8.
3+5+7
Draw a diagram showing the arrangement of the genetic sites
that are involved in the lac operon and briefly summarize the
in
a c.
ut .
role of each site. Explain how the presence of glucose in the
external environment represses transcription of genes in the
lac operon.
9.
8+7
What is translation ? Why aminoacylation of t-RNA is
important before
translation
? Describe
translation process with suitable diagram.
2706
3
each step
of
1 + 4 + 10
[ Turn over
CS/B.Sc.(H), Mol-Bio/SEM-2/GGR-204/2012
10. Define
translation.
Describe
the
sequence
during
the
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p:/
htt
formation of initiation complex. Comment on the role of
ribosome during translation. Give the schematic diagram of
reaction catalyzed by peptidyl transferase in translation.
2+5+3+5
11. What are the basic differences between the repression system
of the lac operon and trp operon ? What are the different
ways by which the trp operon is controlled by the overall
availability of tryptophan ?
5 + 10
pap
=============
wb
er.
4
in
a c.
ut .
2706
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Name : …………………………………………….………………
Roll No. : …………………………………………...……………..
Invigilator’s Signature : ………………………………………..
CS/B.SC (H) MOL-BIO/SEM-2/GGR-204/2011
p:/
2011
GENE EXPRESSION AND GENE REGULATION
Time Allotted : 3 Hours
Full Marks : 70
/qp
The figures in the margin indicate full marks.
Candidates are required to give their answers in their own words
as far as practicable.
ape
GROUP – A
( Multiple Choice Type Questions )
1.
Choose the correct alternatives for any ten of the following :
10 × 1 = 10
The sequence that will be copied in the form of RNA is
called
c)
terminator region
b)
transcription unit
d)
central region.
Number of initiation factors involved in translation are
a)
one
c)
three
b)
two
d)
four.
The function of elongation factor (EFTu) in translation is
a)
binding of amino acyl t-RNA
b)
binding of m-RNA
c)
binding of 30s ribosomal subunit
d)
binding of amino t-RNA.
.in
2706
promoter
t.ac
iii)
a)
bu
ii)
r.w
i)
[ Turn over
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CS/B.SC (H) MOL-BIO/SEM-2/GGR-204/2011
The enzyme responsible for synthesis of RNA molecules
htt
iv)
is
RNA polymerase
b)
peptidyl transferase
c)
RNA synthase
d)
amino peptidase.
p:/
a)
v)
The
binding
of
RNA
polymerase
to
promoter
is
controlled by
c)
vi)
σ factor
b)
α subunit
β subunit
d)
ω subunit.
/qp
a)
When the σ factor is associated with the core enzyme, it
is known as
c)
vii)
holoenzyme
b)
promoter
terminator region
d)
open complex.
ape
a)
The protein which helps to terminate the transcription
is
rho
c)
pre m-NA
b)
t-RNA
d)
small r-RNA.
r.w
a)
viii) Assuming that the level of glucose is low, a mutation in
the repressor of the lac operon in E.coli, preventing
bu
binding of the repressor to the operator, should result in
a)
constitutive expression of the lac operon genes
b)
lack of expression or reduced expression of the lac
t.ac
operon genes under all circumstances
c)
expression of the genes only when lactose is
present
d)
expression of the genes only when lactose is
2706
2
.in
absent.
CS/B.SC (H) MOL-BIO/SEM-2/GGR-204/2011
ix)
pap
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htt
Which of the following is part of an operon ?
a)
structural genes
b)
an operator
c)
a promoter
d)
all of these.
x)
Genes that are always transcribed are called :
a)
constitutive genes
b)
repressor genes
c)
transposons
d)
operons.
xi) Which is not part of the lac operon ?
a)
repressor
b)
activator protein
c)
operator
d)
promotor.
xii) In an operon the location of the regulatory region occurs
..................the structural genes
a)
after
b)
within
c)
before
d)
none of these.
GROUP – B
( Short Answer Type Questions )
wb
er.
Write short notes on any three of the following.
2.
Rho independent transcription termination.
3.
m-RNA processing.
4.
Elongation step of protein synthesis.
5.
Restriction mapping.
6.
Shine Dalgarno sequence.
in
a c.
ut .
GROUP – C
3 × 5 = 15
( Long Answer Type Questions )
Answer any three of the following.
7.
3 × 15 = 45
Define RNA polymerase. Name different kinds of RNA
polymerase
of
eukaryotes
and
describe
their
role
in
transcription. What is Amber, Opal codon ? Define bacterial
1
1
promoter.
3+7+2 +2
2
2
2706
3
[ Turn over
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CS/B.SC (H) MOL-BIO/SEM-2/GGR-204/2011
Define genetic code. How can you prove that genetic code is
htt
8.
triplet. Give the role of different types of antibiotics in protein
synthesis.
p:/
9.
3+7+5
What is meant by a polycistronics transcript, and how is it
related to the lac operon ? What is the normal function of the
/qp
operator site and what is the effect of loss of that function ?
Starting with lactose in the extracellular environment,
describe all the steps that must occur to induce enhanced
ape
expression of the genes in the lac operon.
2+2+2+2+7
10. What are the basic steps of protein synthesis ? Differentiate
the characteristics between prokaryotic and eukaryotic
r.w
ribosome. Give the names of termination triplets and state
the function of release factor in protein synthesis.3 + 5 + 3 + 4
11. How does the interaction between the repressor protein and
bu
the operator site differ between the lac operon and the trp
operon ? Describe two distinctly different ways in which the
t.ac
trp operon is controlled by the overall availability of
1
1
6 + 4 +4
tryptophan.
2
2
4
.in
2706
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Name : ……………………………………………………………
/q
p:/
htt
Roll No. : ………………………………………………………..
Invigilator's Signature : ……………………………………….
CS/B.Sc. (H), Mol.bio)/SEM-2/GGR-204/2010
2010
GENE EXPRESSION AND GENE REGULATION
Time Allotted : 3 Hours
Full Marks : 70
The figures in the margin indicate full marks.
pap
Candidates are required to give their answers in their own words
as far as practicable.
GROUP – A
( Multiple Choice Type Questions )
wb
er.
1.
Choose the correct alternatives for any ten of the following :
i)
ii)
In eukaryotes, the transcription process occurs in
a)
nucleus
c)
plasma membrane
cell wall
d)
cytoplasm.
in
a c.
ut.
2706
b)
The sequence that will be copied in the form of RNA is
called
iii)
10 × 1 = 10
a)
promoter
b)
transcription unit
c)
terminator region
d)
central region.
In eukaryotes, the translation takes place
a)
nucleus
b)
cell wall
c)
plasma membrane
d)
cytoplasm.
[ Turn over
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CS/B.Sc. (H), Mol.bio)/SEM-2/GGR-204/2010
iv)
Number of initiation factors involved in translation is
/q
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htt
v)
a)
one
b)
two
c)
three
d)
four.
The function of elongation factor ( EFTu ) in translation
is
binding of amino acyl t-RNA
b)
binding of m-RNA
c)
binding of 30s ribosomal subunit
d)
binding of amino t-RNA.
The enzyme responsible for synthesis of RNA molecule
is
a)
RNA polymerase
b)
peptidyl transferase
c)
RNA synthase
d)
amino peptidase.
The
binding
controlled by
a)
σ factor
c)
β subunit
wb
er.
vii)
pap
vi)
a)
of
RNA
polymerase
to
promoter
b)
α subunit
d)
ω subunit.
is
is known as
ix)
2706
in
a c.
ut.
viii) When the σ factor is associated with the core enzyme, it
a)
Holoenzyme
b)
Promoter
c)
Terminator region
d)
Open complex.
The protein helps to terminate the transcription is
a)
rho
b)
t-RNA
c)
pre m-NA
d)
small r-RNA.
2
CS/B.Sc. (H), Mol.bio)/SEM-2/GGR-204/2010
x)
Which of the following is the part of an operon ?
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htt
xi)
Structural genes
b)
An operator
c)
A promoter
d)
All of these.
Genes that are always transcribed are called
a)
constitutive genes
b)
repressor genes
c)
transposons
d)
operons.
pap
xii)
a)
Which is not the part of the lac operon ?
Repressor
b)
Activator protein
c)
Operator
d)
Promoter.
wb
er.
a)
GROUP – B
( Short Answer Type Questions )
Write short notes on any three of the following.
m-RNA processing.
3.
Elongation step of protein synthesis.
4.
Wobble hypothesis.
5.
Enhancer sequence.
6.
Restriction modification in bacteria.
2706
3
in
a c.
ut .
2.
3 × 5 = 15
[ Turn over
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CS/B.Sc. (H), Mol.bio)/SEM-2/GGR-204/2010
/q
p:/
htt
GROUP – C
( Long Answer Type Questions )
Answer any three of the following.
7.
3 × 15 = 45
What do you mean by transcription ? Differentiate between
prokaryotic and eukaryotic transcription. Give the location
and function of different classes of RNA molecule.
8.
3+5+7
Define RNA polymerase. Name different kinds of RNA
polymerase
of
eukaryotes
and
describe
their
role
in
transcription. What is bacterial RNA polymerase ? Define
1
9.
pap
bacterial promoter.
1
3 + 7 + 22 + 22
Define genetic code. State the characteristics of genetic code.
Give the role of different types of antibiotics in protein
synthesis.
3+7+5
wb
er.
10. What are the basic steps of protein synthesis ? Differentiate
the
characteristics between prokaryotic and
eukaryotic
ribosome. Give the names of termination triplets and state
the function of release factor in protein synthesis.
3+5+3+4
11. How does the interaction between the repressor protein and
in
a c.
ut.
the operator site differ between the lac operon and the trp
operon ? Describe two distinctly different ways in which the
trp
2706
operon
is controlled by the overall availability of
tryptophan.
6 + 42 + 42
1
4
1
GENE EXPRESSION & GENE REGULATION ( SEMESTER - 2 )
CS/B.Sc (H) (Mol.bio)/SEM-2/GGR-204/09
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1. ………………………………
Signature of Invigilator
2. ………………………………
Reg. No.
Signature of the Officer-in-Charge
Roll No. of the
Candidate
––––––––––––––––––––––––––––––––––––––––––––––––––––––––
CS/B.Sc (H) (Mol.bio)/SEM-2/GGR-204/09
ENGINEERING & MANAGEMENT EXAMINATIONS, JUNE – 2009
Time : 3 Hours ]
pap
GENE EXPRESSION & GENE REGULATION ( SEMESTER - 2 )
[ Full Marks : 70
1.
2.
wb
er.
INSTRUCTIONS TO THE CANDIDATES :
This Booklet is a Question-cum-Answer Booklet. The Booklet consists of 32 pages. The questions of this
concerned subject commence from Page No. 3.
a)
In Group – A, Questions are of Objective type. You have to answer the questions in the
space provided marked 'Answer Sheet'.
b)
7.
8.
9.
in
a c.
ut.
3.
4.
5.
6.
For Groups – B & C you have to answer the questions in the space provided marked ‘Answer
Sheet’. Questions of Group – B are Short answer type. Questions of Group – C are Long answer
type. Write on both sides of the paper.
Fill in your Roll No. in the box provided as in your Admit Card before answering the questions.
Read the instructions given inside carefully before answering.
You should not forget to write the corresponding question numbers while answering.
Do not write your name or put any special mark in the booklet that may disclose your identity, which will
render you liable to disqualification. Any candidate found copying will be subject to Disciplinary Action
under the relevant rules.
Use of Mobile Phone and Programmable Calculator is totally prohibited in the examination hall.
You should return the booklet to the invigilator at the end of the examination and should not take any
page of this booklet with you outside the examination hall, which will lead to disqualification.
Rough work, if necessary is to be done in this booklet only and cross it through.
No additional sheets are to be used and no loose paper will be provided
FOR OFFICE USE / EVALUATION ONLY
Marks Obtained
Group – A
Question
Number
Marks
Obtained
…………………………………………………………
Head-Examiner/Co-Ordinator/Scrutineer
2337 (11/06)
Group – B
Group – C
Total
Marks
Examiner's
Signature
2
CS/B.Sc (H) (Mol.bio)/SEM-2/GGR-204/09
wb
er.
pap
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in
a c.
ut .
2337 (11/06)
3
CS/B.Sc (H) (Mol.bio)/SEM-2/GGR-204/09
ENGINEERING & MANAGEMENT EXAMINATIONS, JUNE – 2009
GENE EXPRESSION & GENE REGULATION
SEMESTER – 2
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Time : 3 Hours ]
www.iqeducation.in
[ Full Marks : 70
GROUP – A
( Objective Type Questions )
1.
Answer the following questions :
(A)
Choose the correct alternatives for any five of the following :
iii)
v)
2337 (11/06)
a)
4 codons
b)
16 codons
c)
20 codons
d)
64 codons.
A dipeptidyl tRNA is produced by catalyzing the enzyme
a)
peptidyl transferase
b)
DNA ligase
c)
RNA polymerase
d)
amino peptidase.
The enzyme responsible for synthesis of RNA molecule is
a)
RNA polymerase
c)
RNA synthase
b)
d)
peptidyl transferase
in
a c.
ut.
iv)
The genetic code contains
wb
er.
ii)
pap
i)
amino peptidase.
Number of eukaryotic RNA polymerase(s) is
a)
one
b)
c)
three
d)
two
four.
Number of initiation factors involved in translation is
a)
one
b)
c)
three
d)
two
four.
5× 1 = 5
4
CS/B.Sc (H) (Mol.bio)/SEM-2/GGR-204/09
vi)
a)
UAA
b)
AUG
c)
UGA
d)
UAG.
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(B)
In translation, the start codon is
Answer any five of the following questions :
vii)
5× 1 = 5
What is polycistronic mRNA ? Give example.
viii) What are enhancers and what are their functions ?
What is HAT and what is its function ?
x)
What are hypersensitive ( HS ) regions in DNA and what are their
functions ?
xi)
pap
ix)
Give examples of some DNA binding domains ( structural motifs ) found in
DNA binding proteins, such as transcription factors.
wb
er.
xii)
What is the function of 5' capping and polyadenylation at the 3' end of newly
synthesized mRNA ?
xiii) What is genomic imprinting ?
GROUP – B
in
a c.
ut .
( Short Answer Type Questions )
Write short notes on any three of the following.
2.
RNA splicing.
3.
Wobble hypothesis.
4.
Rho independent transcription termination.
5.
Clover leaf structure of tRNA.
6.
Role of CpG island in the vertebrate genome.
2337 (11/06)
3 × 5 = 15
www.iqeducation.in
5
CS/B.Sc (H) (Mol.bio)/SEM-2/GGR-204/09
GROUP – C
( Long Answer Type Questions )
Answer any three of the following questions.
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htt
7.
3 × 15 = 45
What do you mean by transcription ? Differentiate between prokaryotic and eukaryotic
transcriptions. Give the location and function of different classes of eukaryotic RNA
polymerases.
8.
3+5+7
What are the basic steps of protein synthesis ? Differentiate the characteristics between
prokaryotic and eukaryotic ribosomes. Give the names of termination triplets and state
the function of release factor in protein synthesis.
pap
9.
3+5+3+4
Define genetic code. State the characteristics of genetic code. Give the role of different
types of antibiotics in protein synthesis.
What is meant by a polycistronic transcript, and how is it related to the lac operon ?
wb
er.
10.
3+7+5
What is the normal function of the operator site and what is the effect of loss of that
function ? Starting with lactose in the extracellular environment, describe all of the
steps that must occur to induce enhanced expression of the genes in the lac operon.
2337 (11/06)
in
a c.
ut.
END
2+2+2+2+7