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biofilm is any group of microorganisms in which cells stick to each other
on a surface. These adherent cells are frequently embedded within a selfproduced matrix of extracellular polymeric substance (EPS). Biofilm
extracellular polymeric substance, which is also referred to as slime
(although not everything described as slime is a biofilm), is a polymeric
conglomeration generally composed of extracellular DNA, proteins, and
polysaccharides. Biofilms may form on living or non-living surfaces
and can be prevalent in natural, industrial and hospital settings.The
microbial cells growing in a biofilm are physiologically distinct from
planktonic cells of the same organism, which, by contrast, are single-cells
that may float or swim in a liquid medium. Microbes form a biofilm in
response to many factors, which may include cellular recognition of
specific or non-specific attachment sites on a surface, nutritional cues,
or in some cases, by exposure of planktonic cells to sub-inhibitory
concentrations of antibiotics. When a cell switches to the biofilm mode
of growth, it undergoes a phenotypic shift in behavior in which large suites
of genes are differentially regulated.