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Low Achieved Candidate explains bacterial digestion Incorrect description of fungal digestion Candidate explains bacterial reproduction Candidate describes fungal reproduction as involving the use of spores The candidate has restated what they wrote on page 2 with the additional information that both can reproduce asexually. Only 3 points = A3 This candidate has described three "Achieved" points; penicillin was most effective, it has the largest no bacterial growth area, and the agar plate should be sterile to prevent contamination such as fungi. Need more than "affect the experiment" or "to keep the experiment honest" as an explanation for sterilising the agar plate. There is no description or explanation on how the agar plate was inoculation Saprophytes are micro-organisms that feed on dead material - not live on / off This candidate has offered no explanation of the temperature changes, nor linked these changes to the life process (respiration) of the micro-organism. No evidence for Achieved or Merit points in this section (b) The candidate has stated two Achieved points: * the bacteria excretes CO2 (a very weak answer, barely acceptable as an Achieved point) * the bacteria is aerobic meaning they use oxygen These additional statements for Question 1 are largely irrelevant (not asked for in the question). They provide no additional evidence towards a higher grade. High Achieved Candidate has explained bacterial digestion, although they have used "excrete" instead of secrete Stated "extra-cellular digestion" DNA is replicated, not split. Binary fission stated Candidate explains fungal reproduction as involving spores which are dispersed. 4 points covered (2 at Merit level) = A4 The candidate has explained how an inoculation loop is used to evenly inoculate the agar plate, i.e. by moving it in an 'S' shape, turning the plate 90 degrees and another 'S' shape drawn = M point Incorrect description / definition This candidate has described how the temperature increased and then decreased (from graph) but has not linked this to the process of respiration The candidate has given a descriptive answer with no explanations. There are three descriptions (1 in part (b) and 2 in part (c) ) and therefore the grade for Question 3 in A3.