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ENZYMATIC HYDROLYSIS OF PROTEINS INTO TAILORED PEPTIDES AND THEIR USE IN DIFFERENT APPLICATIONS Roel Vleeschouwers, W . Dejonghe, K.Elst Flemish Institute for Technological Research [email protected] In recent years, it has been reported that various peptides isolated from chemical or enzymatic hydrolysis of many food proteins or microbial fermentation exert a number of activities affecting the gastrointestinal, endocrine, cardiovascular, immune and nervous systems. Lately these peptide hydrolysates are also used as functional food ingredients in infants, elderly as well as sport nutrition. Also applications in coatings and adhesive products are well known. The traditional hydrochloric acid hydrolysis may form toxicologically critical substances, such as 3-chloro-1,2-propanediol (MCPD) and 1,3-dichloro-2-propanol (DCP), and results in high sodium chloride concentrations in the neutralized products. The enzymatic processing alternative, introduced into industry recently, provides more sustainable and environmentally friendly conditions. Batch-type hydrolysis is the established route to produce protein hydrolysates in food and pharmaceutical industries. However, the hydrolytic efficiency of peptidases decreases due to accumulation of the soluble peptides and free L-amino acids. This is a general phenomenon since it has been observed with various peptidases and different substrates e.g. soy protein, casein, gelatin, and wheat and corn gluten. Thus, high peptidase activities and long incubation times are required, reducing the economic viability of this procedure. Several studies indicated that the enzyme membrane reactor allowed rapid separation of low-molecular-weight peptides from protein hydrolysates, thus avoiding the disadvantages of batch reaction such as inefficient use of enzymes, inconsistent products due to batch-to-batch variation, substrate–product inhibition, low productivity and excessive hydrolysis leading to bitter peptides and amino acids instead of peptides. In addition, immobilization of these peptidases could prevent autolysis and preserving their activity after several runs. This project is intended to develop and optimize an enzymatic hydrolysis process that is combined with an off line (fractionation) or an on line (enzyme membrane reactor) filtration step to obtain tailored peptides from different protein sources. On the one hand we will optimize these two enzymatic hydrolysis processes to increase peptide yield, volumetric productivity as well as the long term use of the enzyme. On the other hand, we will tailor the peptide length and test the functionality of these tailored peptides in different applications such as food (infants, elderly, sport nutrition), feed, nutraceuticals, coatings, adhesives, antimicrobial agents, polymer chemistry (n a t u r a l p o l ya m i d e s ) , p a c k a g i n g m a t e r i a l …. o t h e f i e l d s o f a p p l i c a t i o n s o r t yp e s o f p a r t n e r s yo u a r e l o o k i n g f o r Partners active in production of peptides and use of these peptides in different applications in food (infants, elderly, sport nutrition), feed, nutraceuticals, coatings, adhesives, antimicrobial agents, polymer chemistry (n a t u r a l p o l ya m i d e s ) , p a c k a g i n g m a t e r i a l ….. o Poster + flyer t h e w a y i n w h i c h yo u w a n t t o p r e s e n t yo u r p i t c h ( p o w e r p o i n t , p o s t e r or other)