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Formulation for RPMI-1640 Medium ATCC® 30-2001 Inorganic Salts (g/liter) Ca(NO3)2·4H2O MgSO4 (anhydrous) KCl NaHCO3 NaCl Na2HPO4 (anhydrous) 0.10000 0.04884 0.40000 1.50000 6.00000 0.80000 Amino Acids (g/liter) L-Arginine (free base) L-Asparagine·H2O L-Aspartic Acid L-Cystine·2HCl L-Glutamic Acid L-Glutamine Glycine L-Histidine (free base) Hydroxy-L-Proline L-Isoleucine L-Leucine L-Lysine·HCl L-Methionine L-Phenylalanine L-Proline L-Serine L-Threonine L-Tryptophan L-Tyrosine·2Na·2H2O L-Valine ATCC (American Type Culture Collection) P.O. Box 1549 Manassas, VA 20108 USA www.atcc.org 0.20000 0.05682 0.02000 0.06520 0.02000 0.30000 0.01000 0.01500 0.02000 0.05000 0.05000 0.04000 0.01500 0.01500 0.02000 0.03000 0.02000 0.00500 0.02883 0.02000 Vitamins (g/liter) D-Biotin Choline Chloride Folic Acid myo-Inositol Nicotinamide p-Amino Benzoic Acid D-Pantothenic Acid (hemicalcium) Pyridoxine·HCl Riboflavin Thiamine·HCl Vitamin B-12 0.00020 0.00300 0.00100 0.03500 0.00100 0.00100 0.00025 0.00100 0.00020 0.00100 0.000005 Other (g/liter) D-Glucose Glutathione (reduced) HEPES Phenol Red, Sodium Salt Sodium Pyruvate 4.50000 0.00100 2.38300 0.00500 0.11000 800-638-6597 or 703-365-2700 Fax: 703-365-2750 E-mail: [email protected] - Page 1 of 1 - Or contact your local distributor